FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298946
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG27200.013.82510445
TCGCTCG27200.013.190371514
TATGCCG29850.012.7129548
CGTATGC32550.011.9767346
CTCGTAT34000.010.75567744
CGAAGAT64050.010.6767371
CCGGGAT46200.010.4659471
CTCGGTT27200.010.4120781
CGCGGAT19700.010.3437561
TACTCGT35600.09.8855221
TATCGCG4906.41503E-79.85646910
CCCGATA35450.09.82883569
CGCAGAC59650.09.7851781
ATTAGCG6706.202754E-109.7828416
AGTCACG43200.09.58388128
ACTCGTG37200.09.553061522
TGAAACG41000.09.50786335
CGCTCGT39350.09.46831515
GAAACGA45400.09.4222436
CCGAGAT64150.09.42181
Produced by FastQC (version 0.11.5)