FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32393880
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG20500.013.45403354
GTCGCTA35300.013.21465327
GCGGTAT51500.012.9811611
CGTATCG41750.011.8255523
ATCGAAG70250.011.6810346
GTATCGA30450.011.6293644
CCGACAT63700.011.6194341
TACGCGA23300.011.54506855
CGCGTAA26800.011.0725891
CGATCCA46050.010.2776794
CGCGAAT13650.010.2450921
CCCGTAT32250.010.1532761
CGTATCA28550.010.1373150
CGGTCTA24200.010.13058344
GAACGCT54950.09.50960835
TAGACCG25600.09.44376258
CGCGGAT30350.09.4402891
GCGCGAT27850.09.4304031
GTTACGC31250.09.37054953
CCGGTAT51550.09.3294311
Produced by FastQC (version 0.11.5)