FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36881883
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG108750.041.93402543
CGTATGC111250.040.55758744
TATGCCG113600.039.7489546
CTCGTAT124900.035.600442
ACACGTC129750.035.09598513
GATCGGA128800.034.8109321
TCTCGTA144850.030.43519841
CACGTCT154300.029.51249714
ATGCCGT156100.029.01512747
CACACGT161550.028.35818712
ACGTCTG162750.028.14978215
ATCTCGT161700.027.00769440
TCGGAAG168350.026.5368463
ATCGGAA170500.025.9630072
GATCTCG179500.024.46398439
GCACACG197750.023.96864511
GCCGTCT188250.023.71168949
GTATGCC195050.023.4156645
TGCCGTC197000.023.02622448
CGTCTGA205700.022.43962716
Produced by FastQC (version 0.11.5)