FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36881883
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA41150.021.88130850
GCCGTAT52450.016.64167648
GCGTCGT52600.016.20072412
CGCCGTA58200.015.23470247
TTACGCG25800.015.10232854
GATCGGA67000.014.7314832
TACGCGA30000.013.67429355
CATACGA21450.013.67045715
GCGGTAT53300.012.8201551
CCGTATC70000.012.8143949
GCATACG28000.012.69122714
GTAGATC80300.011.98481132
AGAGCGT76600.011.7554789
CGGCATA30950.011.704473512
GTATCAT86300.011.07288951
AGATCGG80950.010.8712271
CGCGTAA33300.010.7781531
CGTGTAG88750.010.60947216
GTCGTGT87500.010.48812814
ACGCGAC37150.010.48551956
Produced by FastQC (version 0.11.5)