FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38129424
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA57100.034.607350
GCGTCGT60950.032.65094812
GCCGTAT65900.030.72297748
CGCCGTA69600.029.23803347
GATCGGA74400.028.5506552
AGAGCGT87800.022.9800829
AGATCGG93650.022.529471
CCGTATC91050.022.15651949
AGCGTCG92250.022.02056111
TCGCCGT93150.021.366446
GTAGATC94500.021.3130832
CGTCGTG96000.021.26845613
GTCGTGT100100.020.15638214
ATCGGAA103650.020.0349523
TGGTCGC99500.019.7955643
CGTGTAG102100.019.7612116
TCGGAAG108800.019.151054
AAGAGCG109600.019.0380548
GTATCAT107750.018.9135451
TCGTGTA105950.018.84814815
Produced by FastQC (version 0.11.5)