FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17452870
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46200.032.9130443
TATGCCG46650.032.67572846
CGTATGC47700.032.16781244
GATCGGA49100.031.8807531
ACACGTC58050.028.43411413
CTCGTAT54850.027.41107442
ATCGGAA57600.027.2342152
ACGTCTG61750.026.67403415
CACGTCT66100.024.86693214
TCTCGTA61700.024.14620241
ATGCCGT65950.023.68370847
ATCTCGT67950.022.22697340
TCGGAAG72600.021.98293
CGTCTGA78600.021.30215516
CACACGT72250.020.9584712
GCCGTCT75750.020.3958949
AATCTCG36950.019.60509339
CCGTCTT80750.019.38696550
GCACACG80150.019.36327211
TGCCGTC84400.018.83171748
Produced by FastQC (version 0.11.5)