FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17452870
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA21850.031.7352512
GCATACG23400.031.10696814
CATACGA20700.030.99782215
ATACGAG23400.025.35719516
TCGCTCG25850.018.9504214
AGACGGC50000.017.1104559
ACGGCAT43900.016.81644211
AAGACGG50050.016.404498
TTACACG20300.014.275063532
ACTCGTG33950.014.22527522
CGCTCGT34750.014.09685115
CGCAGAC50450.013.9616391
TACGAGA33650.013.94259717
ACGAGAT31950.013.71253318
GACGGCA58050.013.66785810
TACTCGT36600.013.47821321
TCGTGCG35950.013.14601324
CTCGGGT40000.013.1205191
CGAAGAT59400.013.0786671
CGTATCA16250.012.95000750
Produced by FastQC (version 0.11.5)