FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17981977
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG94900.037.10980243
CTCGTAT98200.033.26676642
GATCGGA118450.032.069491
ACACGTC119600.030.98886513
ACGTCTG123800.029.9649615
ATCGGAA127650.029.6205482
ATCTCGT107600.029.42002340
TCTCGTA109450.028.99671741
CGTATGC122850.028.93043344
CACGTCT129300.028.7428514
TATGCCG123550.028.6682346
CACGATC114900.028.43978136
ACGATCT114750.028.24038937
TCGGAAG139800.027.2358363
TCACGAT130350.026.81163235
CCGTCTT134250.026.53823750
GCCGTCT134300.026.5030949
CACACGT142900.026.38743812
GCACACG147400.025.9502711
ATCACGA142600.025.57914434
Produced by FastQC (version 0.11.5)