FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33835533
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA53050.046.63052415
CGGCATA61950.045.0606112
GCATACG64700.044.04561214
ATACGAG68050.032.1944916
ACGGCAT101150.026.87942711
AGACGGC118600.026.1181749
CGTATCA64050.025.85431150
AAGACGG121450.025.4762678
TACGAGA79050.025.22663317
ACGAGAT80250.025.0183118
CGAGATT84250.024.23486719
GCCGTAT70300.023.59881248
CGCCGTA74600.022.56129847
GACGGCA124950.022.30771610
GGCATAC137900.021.56601513
GTCGCCG75800.021.2911345
GCGTCGT79750.020.24906512
TCGCCGT81150.019.93228746
ATACGCG7900.019.65265816
TGGTCGC82100.019.6182443
Produced by FastQC (version 0.11.5)