FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25242013
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4650.014.8735571
TATCGCG5650.014.65468320
TCGTATG38800.012.6255345
CGCGATT9350.012.5749161
CGAAGAT83200.010.6818771
CCGGGAT53550.010.4614851
CGTATGC48200.010.16326946
TATGCCG48650.010.06914248
TTACACG25750.09.78007232
TCGCTCG35200.09.70240714
CTCGTAT49500.09.12975444
ACCCGTC55750.09.03450532
ACACGTC57000.08.89669313
CGGGGAT56300.08.7220331
CCGGTTT30550.08.71600151
GATCGGA50850.08.704761
CGGGTAT17550.08.4728421
CCGCAAT38950.08.4343951
TAAGTCG20050.08.43131520
CGGAAAT45700.08.3236591
Produced by FastQC (version 0.11.5)