FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35594314
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG37050.015.8675493
GTATCGA45050.014.5257744
CCCGTAT45850.013.8902051
TTACACG45750.012.54502232
CGCGAAA18700.012.54053243
ATCGCGC41850.011.54177267
CGTATCA35200.010.93914749
CGTGTTA45450.010.93769359
CGAAGAT132600.010.6614051
GCCGTAT40050.010.31374847
GATCGGA54350.010.2370341
ATCGAAG80900.09.9490486
CTCGGAT87650.09.7412451
CTCCGAT70800.09.63781
CTTGGAT202400.09.5953111
CTCGTAT37400.09.2627551
TCGCGCC53400.09.1764768
CTCGGTT44500.09.1216751
GCGTCGT47450.09.07209811
TAAGTCG36700.09.05956620
Produced by FastQC (version 0.11.5)