FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24398971
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50600.039.54063843
CGTATGC52600.037.51254344
TATGCCG54350.036.81244346
TATCTCG62400.032.4504439
GATCGGA60250.032.418851
AGTCACG63250.032.24353428
ACACGTC64600.031.61328313
CTCGTAT67450.030.27651642
CGCCAAT75100.027.89136933
CACGTCT78050.026.07738114
TCTCGTA80100.025.19356241
ATGCCGT79800.025.1582847
ATCGGAA77450.025.0801262
GTCACGC82650.025.05140729
ACGTCTG82850.024.77475215
ATCTCGT83600.024.46890840
CACACGT89400.023.0363512
TCGGAAG86100.022.9183853
ACGCCAA99100.020.96228232
GCACACG101650.020.90461511
Produced by FastQC (version 0.11.5)