FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24398971
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA23450.022.80250750
GCGTCGT25700.021.61308912
TATCGCG21800.018.20576322
GATCGGA31700.017.8542792
GCCGTAT31950.016.7369748
CGCCGTA33100.016.25970547
AGAGCGT39750.014.2345059
CGTATCG20350.013.5669843
AGCGTCG41900.013.17459111
CCGTATC42200.012.75346549
CCCGTAT25200.012.7362071
AGATCGG42400.012.6974491
GTCGTGT46900.012.65259614
GCGGTAT35650.012.0038311
GTATCGA21450.011.9053364
TGGTCGC45500.011.82722743
ATCGCGG35800.011.76165523
TCGTGTA52000.011.67626615
GTAGATC49100.011.59186832
ATATCGC35600.011.53612721
Produced by FastQC (version 0.11.5)