FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30168691
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG34300.011.53212932
CGCGATT9950.010.5550621
CGCGAAA12550.010.03908143
CGAAGAT102350.09.81651
CGCGAAT11900.09.7079951
CGCGGGT24900.09.7009071
CCGGGAT61000.09.0675481
CCGGAAT51100.08.9745571
CGCGTAT6402.3433131E-78.7519051
CGTTCGC20350.08.42764916
CTCGGGT70850.08.3998531
CTCGGAT74850.08.2783551
TACACGA50750.08.27695133
TCGCTCG36800.08.27458614
CCGAAAT47750.08.2112121
ACGAAAG53450.08.18602536
GTCGTTA20450.08.04337520
TCGGGTC50550.08.0320562
CGACCGT15700.08.0272251
TCGTATG36850.07.977686443
Produced by FastQC (version 0.11.5)