FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30168691
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT59500.010.2942941
TTACACG34750.010.17202732
CGAAGAT101150.09.96556951
TCGCTCG40000.09.53673114
CGCGTAT5301.5351015E-69.2454411
CGACAAT37150.09.044571
CGTTCGC20950.09.02049816
CCGGCAT58450.08.982191
TCGGGTC51650.08.8774162
CGCCGAT51200.08.7501511
CGCGAAA11200.08.74974443
CGCGGAT27450.08.6704591
CTCGGGT69000.08.5218861
CTCGTAT30400.08.5198831
CGCAGAC78300.08.4037211
CGCGTTC23500.08.33975314
CTCGGAT73300.08.16521551
AATCGGG38650.08.1505425
CGGAGAT73050.08.1452461
CGCAAAT38250.08.1439311
Produced by FastQC (version 0.11.5)