FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34016371
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50950.020.78650945
TATGCCG55900.019.31637448
CGTATGC60350.017.7774946
AGTCACG75050.014.70911928
CTCGTAT75700.013.9903944
TGAAACG79150.013.38057735
TCGCTCG48750.013.30385814
ACACGTC83200.012.93676513
GTCACGT85300.012.779839529
GATCGGA81750.012.3911531
ACGATCT86250.012.19910839
AACGATC85800.011.98164238
ACGTCTG90900.011.95476515
CGCTCGT57900.011.6185115
GAAACGA97900.011.52265236
TCTCGTA91100.011.39817943
CACGTCT99050.011.2149114
ATCTCGT92650.011.20749142
ATGCCGT94250.011.12718649
AAACGAT101250.010.86883537
Produced by FastQC (version 0.11.5)