FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34016371
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38350.015.47296850
TCGCTCG53150.014.92893314
CGCCGTA46450.013.21683247
GCCGTAT46250.012.97605648
CGCTCGT61450.012.85680715
CGCAGAC98300.011.3686151
ACTCGTG71550.010.89343922
CCCGATA72550.010.84488569
GTCGCCG54150.010.76243245
TAAGCGA19950.010.71861546
GCGTCGT55400.010.46348912
TACTCGT75100.010.33261121
TCGTGCG76350.010.11971924
CGCGGAT26600.010.1141411
CATACGA16250.09.97847215
CGAAGAT110700.09.9705341
CCGTATC62400.09.89478249
CGCAACC84450.09.80442650
CTCGTAC81000.09.79611917
CGACAAT37200.09.7355941
Produced by FastQC (version 0.11.5)