FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28810865
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26300.030.98730743
CGTATGC33500.024.4487344
TATGCCG34150.023.10150546
CTCGTAT39750.018.759642
ACACGTC48200.018.26787613
GATCGGA49350.016.8179051
CGTATCG30250.014.5550483
GTATCGA30750.013.9880294
GCGGTAT45600.013.8178681
CACGATC53350.013.70825936
TCACGAT60550.013.47615135
CACGTCT66350.013.47487414
CCCGTAT37850.013.4251251
TCTCGTA55000.013.2617541
ACGTCTG66800.013.2272115
GTCGCTA18550.012.59242427
TTACGCG15200.012.252871554
ATCGAAG63300.012.1956846
ACGATCT57500.012.15354537
CACACGT75650.011.81834712
Produced by FastQC (version 0.11.5)