FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28810865
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG30600.014.718543
GTATCGA29600.014.6442744
CCCGTAT32750.013.131471
GCGGTAT42600.012.7183671
TTACGCG15250.012.68303554
ATCGAAG67400.011.3552886
GCGCGAT26300.010.9442031
CGCGTAA22900.010.9425161
TTACACG33100.010.85987832
GTCGCTA22700.010.75728727
TATCGCG8200.010.751101520
TACGCGA18500.010.63729955
GCGGAAT35150.010.3081191
CCGTATC49800.09.99591352
ATCGCGC35450.09.77941767
TAGCGAC20900.09.73640926
CGCGGAT26250.09.6750431
CGACAAT26700.09.511981
CCGACAT49500.09.30367851
TCGAAGG83650.09.2411857
Produced by FastQC (version 0.11.5)