FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44840324
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG64000.014.21778114
CGCGGGT28000.013.2500291
CGCTCGT75950.012.0729115
CGACCGT21450.011.9114481
CGCGGTT23250.011.8924991
CGCAGAC118950.011.8873731
CGCGATT14500.011.34485151
TCGGGTC73450.011.2931692
CGTATCG36850.010.7324963
CTCGGGT92750.010.7170041
TAAGCGA33400.010.4799446
CGCGAAA19550.010.38426743
TTACACG48250.010.22730532
CGCGTAT6907.6397555E-1110.1449491
CGCCTCG93750.09.89268129
CGAAGAT148250.09.8212691
CTCGTAC96100.09.79641917
CCGGGAT68600.09.7959391
TATATCG30400.09.78563835
CTCGGAT95050.09.721221
Produced by FastQC (version 0.11.5)