FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004879289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5537493
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT5336709.637393672551822RNA PCR Primer, Index 5 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC4629578.36040785965779RNA PCR Primer, Index 5 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC3146285.68177693407468RNA PCR Primer, Index 5 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC3111055.618156086156677RNA PCR Primer, Index 5 (100% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC2381094.299942230175279RNA PCR Primer, Index 5 (100% over 44bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1850793.3422886493942294RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC1297152.3424860311335833RNA PCR Primer, Index 5 (100% over 35bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1167152.107722754683392RNA PCR Primer, Index 5 (100% over 45bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG963851.7405891077424387RNA PCR Primer, Index 5 (100% over 39bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC870471.5719568403969086RNA PCR Primer, Index 5 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC835741.5092389281575616No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC782181.4125164582600827RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG697431.259468860728131RNA PCR Primer, Index 5 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC535990.9679289888041392RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT494870.8936715585915864RNA PCR Primer, Index 5 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT436210.7877391447718308RNA PCR Primer, Index 5 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT429270.7752063975521053RNA PCR Primer, Index 5 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT313360.5658878485264RNA PCR Primer, Index 5 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA311550.5626192213696704No Hit
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG305210.5511699969643302RNA PCR Primer, Index 5 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC302740.5467094947117765RNA PCR Primer, Index 5 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT244860.44218566055072217RNA PCR Primer, Index 5 (100% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC234310.4231337177311104RNA PCR Primer, Index 5 (100% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC222290.40142714401625423RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC194760.35171150554953295RNA PCR Primer, Index 5 (100% over 44bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC184710.3335624984085759RNA PCR Primer, Index 5 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG184230.33269568015706746RNA PCR Primer, Index 5 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG182840.3301855189704077RNA PCR Primer, Index 5 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT179670.32446090676773764RNA PCR Primer, Index 5 (100% over 41bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT172930.3122893338194739RNA PCR Primer, Index 5 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT166520.30071369841912216RNA PCR Primer, Index 5 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT163850.29589202189510666RNA PCR Primer, Index 5 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG159410.287873953068654RNA PCR Primer, Index 5 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG146050.26374751173500355RNA PCR Primer, Index 5 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG145690.2630973980463722RNA PCR Primer, Index 5 (100% over 39bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT142520.2573727858437022RNA PCR Primer, Index 5 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT117120.2115036533680494RNA PCR Primer, Index 5 (100% over 43bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC114850.20740432538695763RNA PCR Primer, Index 5 (100% over 44bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC114640.20702509240192266RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT112830.20375646524519309RNA PCR Primer, Index 5 (100% over 43bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC111490.2013365976263988RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC105700.19088060246757874RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG103890.18761197531084914RNA PCR Primer, Index 5 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT101890.18400023259623083RNA PCR Primer, Index 5 (100% over 46bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC87510.158031802478125RNA PCR Primer, Index 5 (100% over 44bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC85100.15367965250700993RNA PCR Primer, Index 5 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA84310.15225301413473571No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC83590.15095278675747312No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA81420.14703404591211222No Hit
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC77640.14020785218148357RNA PCR Primer, Index 5 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC75960.13717398830120417RNA PCR Primer, Index 5 (100% over 44bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC74230.1340498308530593RNA PCR Primer, Index 5 (100% over 42bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC71360.12886698005758201RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC70560.1274222829717347RNA PCR Primer, Index 5 (100% over 35bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC69810.1260678794537528RNA PCR Primer, Index 5 (100% over 42bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC68300.12334101370421596RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG65370.1180498106273001RNA PCR Primer, Index 5 (100% over 45bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT62720.11326425153043082RNA PCR Primer, Index 5 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC62040.11203625900746059No Hit
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG61250.11060962063518635RNA PCR Primer, Index 5 (100% over 45bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC60910.10999562437370124RNA PCR Primer, Index 5 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC60230.108767631850731RNA PCR Primer, Index 5 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT58310.10530035884469742RNA PCR Primer, Index 5 (100% over 46bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC57650.10410848374887335RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCCG57600.10401819018100791RNA PCR Primer, Index 5 (100% over 51bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG56440.10192337940652928RNA PCR Primer, Index 5 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph