FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004879291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20607860
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT253350812.293891748099997RNA PCR Primer, Index 2 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC225174710.926641582386527RNA PCR Primer, Index 2 (100% over 44bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT13555336.577747519635711RNA PCR Primer, Index 2 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG9256794.491873489047383RNA PCR Primer, Index 2 (100% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5461332.650119905705881No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC5271512.55800941970685RNA PCR Primer, Index 2 (100% over 51bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT4369392.120254116633168RNA PCR Primer, Index 2 (100% over 46bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG3527671.7118080188821156RNA PCR Primer, Index 2 (100% over 35bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC3321611.6118170445645497RNA PCR Primer, Index 2 (100% over 42bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3148641.5278830504477419RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT2836571.3764505387750112RNA PCR Primer, Index 2 (100% over 39bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG2187691.0615803872891219RNA PCR Primer, Index 2 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2177781.056771542508538No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1734110.8414799013580255RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT1663360.8071483404875615RNA PCR Primer, Index 2 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1521410.7382668554619451RNA PCR Primer, Index 2 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT1351540.6558371417507689RNA PCR Primer, Index 2 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1293800.6278187060665202RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC1292010.6269501054452039RNA PCR Primer, Index 2 (100% over 44bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG1138140.5522844196340619RNA PCR Primer, Index 2 (100% over 45bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC1082510.5252898651291303RNA PCR Primer, Index 1 (100% over 27bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT1021060.4954711454755613RNA PCR Primer, Index 2 (100% over 43bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT953500.4626875376676666RNA PCR Primer, Index 2 (100% over 46bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC897150.4353436019072334RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG859750.41719518669090333RNA PCR Primer, Index 2 (100% over 38bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT711790.34539733868533656RNA PCR Primer, Index 2 (100% over 39bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG710950.34498972722058474RNA PCR Primer, Index 2 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC691950.33576994408929406RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC664740.3225662441417983RNA PCR Primer, Index 2 (100% over 44bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC645590.31327367324894484RNA PCR Primer, Index 2 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT631680.30652382149335256RNA PCR Primer, Index 2 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG630760.30607738988910055RNA PCR Primer, Index 2 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC620690.3011909048295165RNA PCR Primer, Index 1 (100% over 27bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT616680.2992450453370704RNA PCR Primer, Index 2 (100% over 43bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT602760.2924903410640406RNA PCR Primer, Index 2 (100% over 43bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT567150.2752105264690269RNA PCR Primer, Index 2 (100% over 41bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG558270.2709014909845079RNA PCR Primer, Index 2 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT557010.2702900737873801RNA PCR Primer, Index 2 (100% over 46bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC556040.2698193795959406RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA550400.2670825597611785No Hit
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT548050.265942218163361RNA PCR Primer, Index 2 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG531930.25811996005407645RNA PCR Primer, Index 2 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA531880.2580956974668888No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC479350.23260542336758888No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT408350.19815254956118683RNA PCR Primer, Index 2 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG360490.17492840110520938Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC345150.16748463935605154RNA PCR Primer, Index 2 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG342240.1660725567817328RNA PCR Primer, Index 2 (100% over 45bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC338760.16438388071347534RNA PCR Primer, Index 2 (100% over 44bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC329490.159885597048893RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT328010.15916742446813983RNA PCR Primer, Index 1 (100% over 30bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC319110.15484868394874576RNA PCR Primer, Index 2 (100% over 44bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT317030.1538393603217413RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC307200.14906933568065778RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC307170.1490547781283452RNA PCR Primer, Index 1 (100% over 31bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC295890.14358113845882106RNA PCR Primer, Index 2 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG281040.13637515006410175RNA PCR Primer, Index 2 (100% over 45bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC267670.12988733425013563RNA PCR Primer, Index 2 (100% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG239810.11636822066920097RNA PCR Primer, Index 2 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG238320.11564519557101029RNA PCR Primer, Index 2 (100% over 45bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT232660.11289867070137316RNA PCR Primer, Index 2 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC224950.10915737975704415RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC219080.1063089520212191RNA PCR Primer, Index 1 (100% over 27bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC216470.105042444970026RNA PCR Primer, Index 2 (100% over 44bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT214320.1039991537209589RNA PCR Primer, Index 2 (100% over 43bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT210650.10221827982138854RNA PCR Primer, Index 2 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)