FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12942756
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC222817517.215614665068244RNA PCR Primer, Index 3 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT220778017.058036170966986RNA PCR Primer, Index 3 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT11584908.950875686754815RNA PCR Primer, Index 3 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC7138685.515579525720797RNA PCR Primer, Index 3 (100% over 51bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC4157043.2118661589540896RNA PCR Primer, Index 3 (100% over 42bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT3846382.971840000692279RNA PCR Primer, Index 1 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT3486992.6941634378334878RNA PCR Primer, Index 1 (97% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG3122832.412801415710842RNA PCR Primer, Index 3 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2590242.0013048225586574RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC1433231.107360750677831RNA PCR Primer, Index 3 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT1164400.8996538295244072RNA PCR Primer, Index 1 (97% over 35bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG787560.6084948213502596RNA PCR Primer, Index 3 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT730530.5644315631075792RNA PCR Primer, Index 3 (100% over 41bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACTTAGGCATCTCGT565680.4370630181083534RNA PCR Primer, Index 3 (97% over 46bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC548080.42346467784759284RNA PCR Primer, Index 3 (100% over 39bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACTTAGGC504740.38997876495547007RNA PCR Primer, Index 3 (97% over 39bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACTTAGG492720.3806917166637461RNA PCR Primer, Index 3 (97% over 38bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT421000.3252784801011469RNA PCR Primer, Index 3 (100% over 34bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT392000.3028721239896665RNA PCR Primer, Index 3 (100% over 43bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT387860.2996734234965103RNA PCR Primer, Index 1 (97% over 35bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT382330.2954007631759418RNA PCR Primer, Index 3 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACTTAGGCATCTCGTATGCC374390.28926605739921235RNA PCR Primer, Index 3 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACTT362020.27970858756821193RNA PCR Primer, Index 3 (97% over 35bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC351700.2717350153244023RNA PCR Primer, Index 1 (100% over 27bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT323540.24997767090718545RNA PCR Primer, Index 3 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT300320.23203713335861387RNA PCR Primer, Index 3 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG298790.23085500491549096RNA PCR Primer, Index 3 (100% over 45bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT294320.22740133554244552RNA PCR Primer, Index 3 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC269150.20795416370361924RNA PCR Primer, Index 3 (100% over 42bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC247650.19134255486234927RNA PCR Primer, Index 3 (100% over 44bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT243740.18832155995214622RNA PCR Primer, Index 3 (100% over 43bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC232840.17989986058610702RNA PCR Primer, Index 3 (97% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCG226710.17516362048392164RNA PCR Primer, Index 3 (98% over 51bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC218170.16856533492557535RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT207620.16041405709881265RNA PCR Primer, Index 3 (100% over 46bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT206170.15929373929323862RNA PCR Primer, Index 3 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG190030.14682344316774573RNA PCR Primer, Index 3 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACTTAGGCATCTC186030.14373291129030014RNA PCR Primer, Index 3 (97% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC183280.1416081706245563RNA PCR Primer, Index 1 (100% over 31bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT178650.13803087997641306RNA PCR Primer, Index 3 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT172660.1334028084899383RNA PCR Primer, Index 3 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC166310.12849658913449347RNA PCR Primer, Index 3 (100% over 44bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT164520.12711357611933655RNA PCR Primer, Index 3 (97% over 43bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC162950.1259005423574392RNA PCR Primer, Index 3 (100% over 42bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAGCTCGT157740.12187512458706631RNA PCR Primer, Index 3 (97% over 46bp)
CGTGGTGGAATTCTCAGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT154910.11968857328377355RNA PCR Primer, Index 3 (97% over 46bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT154540.11940269908510984RNA PCR Primer, Index 1 (97% over 35bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC150260.11609582997624307RNA PCR Primer, Index 3 (100% over 44bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT148100.11442694276242248RNA PCR Primer, Index 3 (100% over 43bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG147520.11397881564019285RNA PCR Primer, Index 3 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC147090.11364658346336746No Hit
TCCCGTGGTGGAATTCTCGGGTGCCTAGGAACTCCAGTCACTTAGGCATCT145490.11241037071238924RNA PCR Primer, Index 3 (97% over 43bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC141580.10938937580218618RNA PCR Primer, Index 3 (100% over 42bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC139920.10810680507304628RNA PCR Primer, Index 3 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAACTC139860.10806044709488459RNA PCR Primer, Index 3 (97% over 44bp)
ACCACGTTCCCGTGGTGGAATTCTCTGGTGCCAAGGAACTCCAGTCACTT136300.10530987372395803RNA PCR Primer, Index 3 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC913500.047.52607745
GCATTAA253.843599E-545.097491
TCCCACA1450.045.097491
CAATGTT454.0199666E-1044.809596
TACCGCC454.0199666E-1044.809595
ATAATTG502.3646862E-1144.8092429
TATAATT502.3646862E-1144.8092428
CCTACAC950.044.80907410
ACAGAAC1050.044.80907411
CCTTTAA1150.044.8090711
GAGTACC454.0199666E-1044.8075142
CCCCAAC407.0886017E-944.8075142
CTATTCG207.1861234E-444.8075142
CCCATAT650.044.8075142
AATTACA1450.044.807512
CCCTATA207.1873545E-444.8059543
TTCCATA253.9936498E-544.8059543
CCTATAG253.9936498E-544.8059544
TGGTGAT207.1873545E-444.8059543
GACGCGG407.0995156E-944.7988623
Produced by FastQC (version 0.11.5)