FastQCFastQC Report
Fri 10 May 2024
EGAF00004879294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15815170
Total Bases804.3 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT266586216.856360064419164RNA PCR Primer, Index 4 (100% over 43bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC264703916.737341425985303RNA PCR Primer, Index 4 (100% over 42bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG13678768.64913876992786RNA PCR Primer, Index 4 (100% over 45bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT4719852.9843814514798135RNA PCR Primer, Index 4 (100% over 41bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT4498192.8442248802889885RNA PCR Primer, Index 3 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA3336782.109860342949206RNA PCR Primer, Index 4 (100% over 36bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3044061.9247722281834467RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC2927131.8508368863565803RNA PCR Primer, Index 4 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC2027161.2817819852711037RNA PCR Primer, Index 4 (100% over 50bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA1483720.9381625363495935RNA PCR Primer, Index 4 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1229830.7776267975620875RNA PCR Primer, Index 4 (100% over 35bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC942100.595693881254517RNA PCR Primer, Index 4 (100% over 44bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA868510.5491626077999794RNA PCR Primer, Index 4 (100% over 40bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACTGACCAATCTCG707280.4472161854725558RNA PCR Primer, Index 4 (97% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC606630.38357475765356935RNA PCR Primer, Index 4 (100% over 44bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACTGACCA575870.36412507737823874RNA PCR Primer, Index 4 (97% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG548730.3469643386697709RNA PCR Primer, Index 4 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC540110.3415138756017166RNA PCR Primer, Index 4 (100% over 38bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC510780.3229683904757268RNA PCR Primer, Index 4 (100% over 42bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC507370.3208122328119141RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC502260.3175811578376963RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG488450.3088490354514052RNA PCR Primer, Index 4 (100% over 45bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT487080.3079827785600787RNA PCR Primer, Index 4 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC485530.3070027068947093RNA PCR Primer, Index 4 (100% over 42bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACTGAC483010.30540930005810873RNA PCR Primer, Index 4 (97% over 37bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC470540.29752446543413696RNA PCR Primer, Index 4 (100% over 44bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG432200.273281918563RNA PCR Primer, Index 4 (100% over 35bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT407460.25763871017510404RNA PCR Primer, Index 4 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG374200.23660826914917765RNA PCR Primer, Index 4 (100% over 45bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT360280.2278065932898603RNA PCR Primer, Index 4 (100% over 43bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT354190.22395586010140897RNA PCR Primer, Index 4 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC353610.22358912360727073RNA PCR Primer, Index 4 (100% over 42bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT328590.20776887001530808RNA PCR Primer, Index 4 (100% over 41bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC327860.20730728787613412No Hit
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACTGA321390.20321627905359219RNA PCR Primer, Index 4 (97% over 36bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC311330.19685529779319477RNA PCR Primer, Index 4 (100% over 42bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT305850.1933902702278888RNA PCR Primer, Index 4 (100% over 41bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC283890.17950486779465538RNA PCR Primer, Index 4 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA266310.1683889581964658RNA PCR Primer, Index 1 (100% over 32bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG264970.16754167043414647RNA PCR Primer, Index 4 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT255260.1614019956788324RNA PCR Primer, Index 4 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC248930.15739950945832387RNA PCR Primer, Index 4 (100% over 44bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG247910.1567545590720808RNA PCR Primer, Index 4 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT240910.1523284289704126RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACTGACCAATCT227360.1437607057021834RNA PCR Primer, Index 4 (97% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACTGACCAATCT224140.14172468585541603RNA PCR Primer, Index 4 (97% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC221420.1400048181587678RNA PCR Primer, Index 4 (100% over 44bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT217820.1377285226779099RNA PCR Primer, Index 4 (100% over 41bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACTGACCAATC205070.12966664284987137RNA PCR Primer, Index 4 (97% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC197350.12478525365203157RNA PCR Primer, Index 4 (98% over 50bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC193460.1223255899241045RNA PCR Primer, Index 4 (100% over 42bp)
TCCCGTGGTGGAATTCTCGGGTGCCTAGGAACTCCAGTCACTGACCAATC191320.12097245872159453RNA PCR Primer, Index 4 (97% over 42bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAACT181630.11484543005228524RNA PCR Primer, Index 4 (97% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC181460.11473793832124472RNA PCR Primer, Index 4 (100% over 42bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT176950.11188624592716992RNA PCR Primer, Index 4 (100% over 43bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC175910.11122864945492207RNA PCR Primer, Index 4 (100% over 42bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT172040.1087816318129998RNA PCR Primer, Index 4 (100% over 43bp)
CCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT168670.10665076632119666RNA PCR Primer, Index 4 (100% over 43bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT164870.10424800998029106RNA PCR Primer, Index 3 (100% over 34bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT163340.10328058440092645RNA PCR Primer, Index 4 (100% over 41bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC161240.10195274537042598RNA PCR Primer, Index 4 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)