FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004879297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611128
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT77923610.23811450812547RNA PCR Primer, Index 6 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC5169726.792317774710924RNA PCR Primer, Index 6 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC3705044.867924964604458RNA PCR Primer, Index 6 (100% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC3574144.695939944775597RNA PCR Primer, Index 6 (100% over 44bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC3442584.523087773586254RNA PCR Primer, Index 5 (97% over 35bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2673443.512541110857681RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG2028192.664769269417096RNA PCR Primer, Index 6 (100% over 45bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1419081.864480534291369RNA PCR Primer, Index 6 (100% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT1370261.800337610929681RNA PCR Primer, Index 6 (100% over 39bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC1274701.6747846048575192RNA PCR Primer, Index 6 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1214151.595230036861816No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG1122271.4745120565571883RNA PCR Primer, Index 6 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC968451.2724132349370552RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT789061.0367188674267467RNA PCR Primer, Index 6 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT668380.8781615550283741RNA PCR Primer, Index 6 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG659620.8666520915165269RNA PCR Primer, Index 6 (100% over 34bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT622140.8174084051667505RNA PCR Primer, Index 6 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG547190.718934171124175RNA PCR Primer, Index 6 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC545940.7172918390020506RNA PCR Primer, Index 6 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA444980.5846439581623117No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT422740.5554235850454755RNA PCR Primer, Index 6 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA359880.4728339872880866RNA PCR Primer, Index 6 (100% over 37bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC334960.44009245410141573RNA PCR Primer, Index 6 (100% over 36bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG313640.4120808374264629RNA PCR Primer, Index 6 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC311950.40986040439735083RNA PCR Primer, Index 6 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC303350.3985611593971354RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC293970.3862370991527143RNA PCR Primer, Index 6 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT290420.3815728759258812RNA PCR Primer, Index 6 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG287910.37827507302465546RNA PCR Primer, Index 6 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG282920.3717188831931351RNA PCR Primer, Index 6 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT279010.36658166831513017RNA PCR Primer, Index 6 (100% over 41bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT277590.36471597902439695RNA PCR Primer, Index 6 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT253890.3335773619889194RNA PCR Primer, Index 6 (100% over 46bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT253040.3324605761458749RNA PCR Primer, Index 6 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG239690.31492046908158683RNA PCR Primer, Index 6 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT183080.24054253193481964RNA PCR Primer, Index 6 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT178540.23457758166726406RNA PCR Primer, Index 6 (100% over 43bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG172850.22710168584735405RNA PCR Primer, Index 6 (100% over 45bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC172360.2264578916554813RNA PCR Primer, Index 6 (100% over 44bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT167460.22001994973675384RNA PCR Primer, Index 6 (100% over 43bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT159610.209706104009813RNA PCR Primer, Index 6 (100% over 39bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC154520.20301852760852265RNA PCR Primer, Index 1 (100% over 31bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC146410.19236307680017994RNA PCR Primer, Index 6 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC132940.17466530585216802RNA PCR Primer, Index 1 (100% over 27bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC132450.1740215116602953RNA PCR Primer, Index 6 (100% over 44bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC132140.17361421329400845RNA PCR Primer, Index 6 (100% over 42bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC125340.16467992654965205RNA PCR Primer, Index 1 (100% over 33bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC125020.1642594895263882RNA PCR Primer, Index 6 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA124230.16322153562520564No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA119380.15684928699136316No Hit
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC117140.15390622782851635RNA PCR Primer, Index 6 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG110440.1451033276539299RNA PCR Primer, Index 6 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC109120.14336902493296658No Hit
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC105900.13913837738637425RNA PCR Primer, Index 6 (100% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG104650.1374960452642499RNA PCR Primer, Index 6 (100% over 45bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT102210.1342902129618632RNA PCR Primer, Index 6 (100% over 46bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC102000.13401430116534632RNA PCR Primer, Index 6 (100% over 42bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG99510.13074277557807462RNA PCR Primer, Index 6 (100% over 45bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT96180.12636760280473538RNA PCR Primer, Index 6 (100% over 43bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC94740.12447563620004813RNA PCR Primer, Index 5 (97% over 35bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC92160.1210858626999835RNA PCR Primer, Index 6 (100% over 44bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG89830.11802455562434373RNA PCR Primer, Index 6 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC87980.11559390408359971RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC83840.11015450009512388RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC83560.10978661769976802RNA PCR Primer, Index 6 (100% over 44bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC78870.10362458757755749RNA PCR Primer, Index 6 (100% over 42bp)
GGAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC77840.10227130590892704RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG77380.10166692768798528RNA PCR Primer, Index 6 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)