FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16769440
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC265817715.851316442290262RNA PCR Primer, Index 7 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT233198713.906170987224378RNA PCR Primer, Index 7 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT13397367.989151694988025RNA PCR Primer, Index 7 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4007702.3898830253127117RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC3810432.2722464196776997RNA PCR Primer, Index 7 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3139571.8721972826761062RNA PCR Primer, Index 2 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2524211.505244063009856RNA PCR Primer, Index 2 (100% over 34bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2228641.3289889227070195RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC2187791.304629134902537RNA PCR Primer, Index 7 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC1232080.7347174383879247RNA PCR Primer, Index 7 (100% over 39bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1226160.7311872072054881RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT1064290.634660429924911RNA PCR Primer, Index 7 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG1064150.6345769447280292RNA PCR Primer, Index 7 (100% over 45bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1061460.632972836302226RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT1014250.6048204352679636RNA PCR Primer, Index 7 (100% over 46bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG953080.5683433674589013RNA PCR Primer, Index 7 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT941900.5616764781650431RNA PCR Primer, Index 7 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG935220.5576930416281044RNA PCR Primer, Index 7 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT858440.5119073743667052RNA PCR Primer, Index 7 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC832420.4963910542033604RNA PCR Primer, Index 7 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC746090.4449105038689426No Hit
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACCAGATCATCTCGT624420.37235590455018175RNA PCR Primer, Index 7 (97% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT620720.37014951006115887RNA PCR Primer, Index 7 (100% over 41bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT594980.354800160291578RNA PCR Primer, Index 7 (100% over 46bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT512690.3057287542100392RNA PCR Primer, Index 7 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC454290.27090350065356983RNA PCR Primer, Index 2 (100% over 34bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT452060.26957370073180736RNA PCR Primer, Index 7 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT448740.2675939089200355RNA PCR Primer, Index 7 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG445040.2653875144310126RNA PCR Primer, Index 7 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC439700.2622031504928012RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACCAGATC431800.2574922000973199RNA PCR Primer, Index 7 (97% over 39bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG428730.2556614889942658RNA PCR Primer, Index 7 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG416040.24809415221975212RNA PCR Primer, Index 7 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACC394940.23551174040397296RNA PCR Primer, Index 2 (97% over 34bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC383020.22840357221231003RNA PCR Primer, Index 7 (100% over 42bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT374740.2234660191395777RNA PCR Primer, Index 7 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC374120.22309629898195765RNA PCR Primer, Index 7 (100% over 44bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC353650.21088957055214724RNA PCR Primer, Index 7 (100% over 44bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT349120.2081882281101814RNA PCR Primer, Index 7 (100% over 43bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG340240.20289288133652647RNA PCR Primer, Index 7 (100% over 45bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT326470.19468151590035207RNA PCR Primer, Index 7 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT323580.19295814290757474RNA PCR Primer, Index 7 (100% over 43bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT304870.18180094266713737RNA PCR Primer, Index 7 (100% over 46bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT303760.1811390243204305RNA PCR Primer, Index 7 (100% over 46bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT286120.17061988951330515RNA PCR Primer, Index 7 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC277220.1653126162829528RNA PCR Primer, Index 7 (100% over 42bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG274240.16353557423503706RNA PCR Primer, Index 7 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACC270790.16147826045473196RNA PCR Primer, Index 2 (97% over 34bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG265550.15835352880000764RNA PCR Primer, Index 7 (100% over 45bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC265440.1582879332881718RNA PCR Primer, Index 7 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT262430.15649300155520995RNA PCR Primer, Index 7 (100% over 46bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC253630.1512453606083447RNA PCR Primer, Index 7 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCCG243220.14503763989733706RNA PCR Primer, Index 7 (98% over 51bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT242360.14452480225934794RNA PCR Primer, Index 7 (100% over 43bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC241010.14371976643227205RNA PCR Primer, Index 7 (100% over 44bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC222540.13270568367220373RNA PCR Primer, Index 7 (97% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC221100.1318469787899894RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGTAATTCTCGGGTGCCAAGGAACTCC219300.13077359768722152RNA PCR Primer, Index 1 (96% over 27bp)
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT215930.12876398973370606RNA PCR Primer, Index 7 (100% over 46bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACCAGATCATCTC215930.12876398973370606RNA PCR Primer, Index 7 (97% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC214520.12792317453653787RNA PCR Primer, Index 7 (100% over 44bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC212390.12665300689826256RNA PCR Primer, Index 2 (100% over 34bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT206250.1229915846921543RNA PCR Primer, Index 7 (100% over 46bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG205860.12275901878655458RNA PCR Primer, Index 7 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC203380.12128013815607439No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA197690.1178870612256581No Hit
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT196210.11700450343004895RNA PCR Primer, Index 7 (100% over 43bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC194750.11613387209113721RNA PCR Primer, Index 7 (100% over 42bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT192730.11492929996469768RNA PCR Primer, Index 7 (100% over 43bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC189950.11327152248375616RNA PCR Primer, Index 1 (100% over 31bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC180330.10753489681229667RNA PCR Primer, Index 7 (100% over 44bp)
CCCCCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT173000.10316385043269184RNA PCR Primer, Index 7 (100% over 43bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG172530.10288357870030244RNA PCR Primer, Index 7 (100% over 45bp)
CCCGCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT169380.10100516177045864RNA PCR Primer, Index 7 (100% over 43bp)
CCCCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT167850.10009278783310593RNA PCR Primer, Index 7 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)