FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15928424
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC262824916.500370658139186RNA PCR Primer, Index 8 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT248213715.583067100674869RNA PCR Primer, Index 8 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT13377928.398771906122036RNA PCR Primer, Index 8 (100% over 46bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC4367702.742079191262111RNA PCR Primer, Index 8 (100% over 42bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC4202312.638245943227026RNA PCR Primer, Index 5 (100% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT3345382.1002580041817067RNA PCR Primer, Index 8 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG2771391.739902202502897RNA PCR Primer, Index 8 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2691071.6894766236760148RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC1968471.2358222006144488RNA PCR Primer, Index 8 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA1455240.9136120434764922RNA PCR Primer, Index 8 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT1188600.7462131846816734RNA PCR Primer, Index 8 (100% over 36bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG941070.5908117463472846RNA PCR Primer, Index 8 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG838310.5262981447505416RNA PCR Primer, Index 8 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT736480.4623684050600361RNA PCR Primer, Index 8 (100% over 41bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT718600.45114318905624307RNA PCR Primer, Index 8 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT686620.4310658731836873RNA PCR Primer, Index 8 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT678740.42611874219320134RNA PCR Primer, Index 8 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG651630.4090988537221259RNA PCR Primer, Index 8 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACACTTGAATCTCGT624280.3919282912107312RNA PCR Primer, Index 8 (97% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC569610.35760600044298163RNA PCR Primer, Index 8 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT544880.3420802962050734RNA PCR Primer, Index 8 (100% over 46bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACACTTGA520560.3268119934527107RNA PCR Primer, Index 8 (97% over 39bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT499120.31335177918418045RNA PCR Primer, Index 8 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC484460.30414810655467234RNA PCR Primer, Index 1 (100% over 27bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT482100.30266647849153183RNA PCR Primer, Index 8 (100% over 43bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC479190.3008395557526595RNA PCR Primer, Index 1 (100% over 33bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTG475510.29852922046776253RNA PCR Primer, Index 8 (100% over 38bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC464700.29174261056837764No Hit
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACACTTG426180.267559427097119RNA PCR Primer, Index 8 (97% over 38bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC374550.23514567417341475RNA PCR Primer, Index 8 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT372150.23363893377022107RNA PCR Primer, Index 8 (100% over 46bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC354670.22266484116696042RNA PCR Primer, Index 8 (100% over 42bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT352880.22154106394957845RNA PCR Primer, Index 8 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT347700.2182890159126854RNA PCR Primer, Index 8 (100% over 43bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT332490.2087400486074454RNA PCR Primer, Index 8 (100% over 46bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG330650.2075848809649969RNA PCR Primer, Index 8 (100% over 45bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC320210.20103056021110438RNA PCR Primer, Index 8 (100% over 42bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC319890.20082966149067855RNA PCR Primer, Index 8 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG319600.20064759702529264RNA PCR Primer, Index 8 (100% over 45bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT318240.1997937774634829RNA PCR Primer, Index 8 (100% over 36bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG310930.19520449731875547RNA PCR Primer, Index 8 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACACTT266980.16761231368527107RNA PCR Primer, Index 8 (97% over 37bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT266300.1671854039043662RNA PCR Primer, Index 8 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT254300.15965170188839775RNA PCR Primer, Index 8 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC246210.15457273111263237RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC241280.151477635201072RNA PCR Primer, Index 8 (97% over 44bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC239690.15047941968395617RNA PCR Primer, Index 8 (100% over 42bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT229230.14391254276003704RNA PCR Primer, Index 8 (100% over 43bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC216270.1357761445827911RNA PCR Primer, Index 8 (100% over 44bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT214810.13485954417084828RNA PCR Primer, Index 8 (100% over 43bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT213260.13388644099378572RNA PCR Primer, Index 8 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG211100.13253037463091139RNA PCR Primer, Index 8 (100% over 45bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCCG208380.13082273550729187RNA PCR Primer, Index 8 (98% over 51bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACACTTGAATCTC207410.1302137612610011RNA PCR Primer, Index 8 (97% over 44bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT202710.1272630613047468RNA PCR Primer, Index 8 (100% over 43bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC196440.12332670200140329RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG194660.12220920286903462RNA PCR Primer, Index 8 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT179190.11249700535344866RNA PCR Primer, Index 8 (100% over 46bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT178750.11222076961286313RNA PCR Primer, Index 8 (100% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC176290.11067636069958962RNA PCR Primer, Index 8 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCG174040.10926379157159553RNA PCR Primer, Index 8 (100% over 45bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT171350.10757498670301595RNA PCR Primer, Index 8 (97% over 43bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC165620.10397764399039103RNA PCR Primer, Index 8 (100% over 44bp)
CCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC164130.10304220932340825RNA PCR Primer, Index 8 (100% over 44bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT163400.10258390911743685RNA PCR Primer, Index 8 (100% over 43bp)
CGTGGTAGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGT160610.1008323233987242RNA PCR Primer, Index 8 (97% over 46bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC160430.10071931786848466RNA PCR Primer, Index 8 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)