FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746922
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC243212916.49245178078517RNA PCR Primer, Index 9 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT241829816.39866271754879RNA PCR Primer, Index 9 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT12215378.28333532923006RNA PCR Primer, Index 9 (100% over 46bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC4329492.935860106942995RNA PCR Primer, Index 9 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3601172.4419807740218604RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC2912421.9749341591418195RNA PCR Primer, Index 9 (100% over 37bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2667281.8087028601629547RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC2535751.719511366507533RNA PCR Primer, Index 9 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC1793431.2161385270770402RNA PCR Primer, Index 9 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC1497351.0153644265562671RNA PCR Primer, Index 9 (100% over 37bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1145840.777002821334513RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1029060.6978134148943081RNA PCR Primer, Index 9 (100% over 36bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG845210.5731433311981985RNA PCR Primer, Index 9 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC730790.49555425871242825RNA PCR Primer, Index 9 (100% over 37bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG681240.4619540267453778RNA PCR Primer, Index 9 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT666300.4518230990846768RNA PCR Primer, Index 9 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT625640.4242512437510689RNA PCR Primer, Index 9 (100% over 46bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGATCAGATCTCGT587640.39848315465423906RNA PCR Primer, Index 9 (97% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT574070.3892812344162395RNA PCR Primer, Index 9 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG553540.3753596852278733RNA PCR Primer, Index 9 (100% over 45bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGATC519470.35225655902974196RNA PCR Primer, Index 9 (97% over 37bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC482680.3273089801383638RNA PCR Primer, Index 9 (100% over 37bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT479760.3253289059235548RNA PCR Primer, Index 9 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT469400.3183037111066296RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT465220.3154692213059783RNA PCR Primer, Index 9 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC457800.31043766285601837RNA PCR Primer, Index 9 (100% over 44bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG419840.28469669806350095RNA PCR Primer, Index 6 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGATC379300.2572062156428304RNA PCR Primer, Index 9 (97% over 37bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT362560.24585469428806905RNA PCR Primer, Index 9 (100% over 36bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT338150.22930208758139495RNA PCR Primer, Index 9 (100% over 46bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT334170.22660321930230592RNA PCR Primer, Index 9 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT325360.22062909127748825RNA PCR Primer, Index 9 (100% over 43bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC324770.2202290077888796RNA PCR Primer, Index 9 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG316610.2146956497091393RNA PCR Primer, Index 9 (100% over 45bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT306600.20790779255494807RNA PCR Primer, Index 9 (100% over 46bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC301860.20469356249392245RNA PCR Primer, Index 9 (97% over 44bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC283540.19227063111881923RNA PCR Primer, Index 9 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG282030.19124668863102415RNA PCR Primer, Index 9 (100% over 45bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC280470.1901888407628385RNA PCR Primer, Index 9 (100% over 37bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC256660.17404309862085118RNA PCR Primer, Index 9 (100% over 44bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT252140.17097805223354406RNA PCR Primer, Index 9 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG251840.17077461995120066RNA PCR Primer, Index 9 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGATC237800.16125398913752986RNA PCR Primer, Index 9 (97% over 37bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC232730.15781598356592652RNA PCR Primer, Index 1 (100% over 33bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTCGT225100.1526420225183262RNA PCR Primer, Index 9 (97% over 46bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC222180.15066194830351715No Hit
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC217660.14759690191621005RNA PCR Primer, Index 9 (100% over 37bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT214880.1457117627664946RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACGATCAGATCTC204390.13859841396055395RNA PCR Primer, Index 9 (97% over 44bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT202700.13745241210335282RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTC201520.13665224512613547RNA PCR Primer, Index 9 (97% over 44bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT201080.1363538777786985RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAACTC198940.13490272749798227RNA PCR Primer, Index 9 (97% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC191740.13002035272174084RNA PCR Primer, Index 1 (100% over 31bp)
TTCCCTTTGTCATCCTATGCCTGTGTAATTCTCGGGTGCCAAGGAACTCC181780.1232664009479402RNA PCR Primer, Index 1 (96% over 27bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC180140.12215430447112964RNA PCR Primer, Index 9 (100% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG176800.11988942506103986RNA PCR Primer, Index 9 (98% over 51bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG174230.11814668850896477RNA PCR Primer, Index 9 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT172450.11693965696706066RNA PCR Primer, Index 9 (100% over 43bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT171720.11644463841335839RNA PCR Primer, Index 9 (100% over 46bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG170210.11542069592556331RNA PCR Primer, Index 9 (100% over 45bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT168490.11425435084012785RNA PCR Primer, Index 9 (97% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC162860.11043660500815017RNA PCR Primer, Index 9 (100% over 44bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT160220.10864640092352831RNA PCR Primer, Index 9 (100% over 43bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT157460.1067748239259691RNA PCR Primer, Index 9 (100% over 43bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG152200.1032079779088816RNA PCR Primer, Index 9 (100% over 45bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC149930.10166867363914991RNA PCR Primer, Index 9 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)