FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964716
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT277730517.396519925565855RNA PCR Primer, Index 11 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC267184116.7359131224132RNA PCR Primer, Index 11 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT12759407.9922499091120685RNA PCR Primer, Index 11 (100% over 46bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC4934123.0906406352609093RNA PCR Primer, Index 11 (100% over 42bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG3670742.299282993822126RNA PCR Primer, Index 2 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT2984301.869309795426364RNA PCR Primer, Index 11 (100% over 37bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2820111.766464245276897RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT2578541.6151493080114925RNA PCR Primer, Index 11 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC1985121.2434421006925522RNA PCR Primer, Index 11 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC1703551.0670719103302557RNA PCR Primer, Index 11 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC1062060.6652545526021258RNA PCR Primer, Index 6 (97% over 36bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG902050.5650272764012839RNA PCR Primer, Index 11 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT875400.5483342140254797RNA PCR Primer, Index 11 (100% over 41bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG729730.45708924605987356RNA PCR Primer, Index 11 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT673450.4218365049525466RNA PCR Primer, Index 11 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC635720.3982031374689033RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT622520.389934903946929RNA PCR Primer, Index 11 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGGCTACATCTCGT604080.37838443226926183RNA PCR Primer, Index 11 (97% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT582900.3651176757544575RNA PCR Primer, Index 11 (100% over 46bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGGCTAC564700.3537175355953717RNA PCR Primer, Index 11 (97% over 39bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT564350.3534983021307739RNA PCR Primer, Index 11 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG546700.34244267170177034RNA PCR Primer, Index 11 (100% over 45bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT541440.33914790591952904RNA PCR Primer, Index 11 (100% over 46bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC476850.2986899359813228RNA PCR Primer, Index 11 (100% over 39bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC463370.29024631568767023RNA PCR Primer, Index 11 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT438440.27463062919503234RNA PCR Primer, Index 11 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG427890.26802230619072714RNA PCR Primer, Index 6 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGGCT395680.24784656363445487RNA PCR Primer, Index 11 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC381230.23879535345320266No Hit
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT376950.23611444137183524RNA PCR Primer, Index 11 (100% over 43bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC376500.2358325697744952RNA PCR Primer, Index 6 (97% over 36bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC374910.23483662346389372RNA PCR Primer, Index 11 (100% over 42bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT351220.2199976498172595RNA PCR Primer, Index 11 (100% over 46bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT346740.21719146147040763RNA PCR Primer, Index 11 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC329290.20626110730688854RNA PCR Primer, Index 11 (100% over 42bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG325670.20399360690161983RNA PCR Primer, Index 11 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT319410.2000724597919562RNA PCR Primer, Index 11 (100% over 46bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC319140.19990333683355221RNA PCR Primer, Index 11 (100% over 44bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT317730.19902013916188677RNA PCR Primer, Index 11 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC295760.18525854139841885RNA PCR Primer, Index 11 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG292630.18329796784358707RNA PCR Primer, Index 11 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG268720.16832119030491993RNA PCR Primer, Index 11 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGGCT262570.1644689451412728RNA PCR Primer, Index 11 (97% over 37bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC253570.1588315131944721RNA PCR Primer, Index 11 (100% over 42bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC241230.15110196761408096RNA PCR Primer, Index 1 (100% over 33bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT230170.14417419013279034RNA PCR Primer, Index 11 (100% over 43bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT226820.14207581268592565RNA PCR Primer, Index 11 (100% over 43bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC225700.14137426559921268RNA PCR Primer, Index 11 (97% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACGGCTACATCTC223870.14022798777002984RNA PCR Primer, Index 11 (97% over 44bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT213590.13378878772412864RNA PCR Primer, Index 11 (100% over 43bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC208800.13078842116577583RNA PCR Primer, Index 1 (100% over 31bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC200180.12538901412339562RNA PCR Primer, Index 11 (100% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG193730.1213488545615218RNA PCR Primer, Index 11 (98% over 51bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT191840.12016499385269365RNA PCR Primer, Index 11 (100% over 46bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT188240.11791002107397339RNA PCR Primer, Index 11 (97% over 43bp)
TCCCGTGGTGGAATTCTCGGGTGCCTAGGAACTCCAGTCACGGCTACATCT186640.11690781095009771RNA PCR Primer, Index 11 (97% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT182210.11413294166961693RNA PCR Primer, Index 11 (100% over 43bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT181580.11373832143334087RNA PCR Primer, Index 11 (100% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC179490.11242918445902826RNA PCR Primer, Index 11 (100% over 44bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT176220.11038091751835737RNA PCR Primer, Index 11 (100% over 43bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG173260.10852682878918736RNA PCR Primer, Index 11 (100% over 45bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC171940.10770000543698993RNA PCR Primer, Index 11 (100% over 42bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG169910.10642845134232266RNA PCR Primer, Index 11 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAACTC160610.10060310499729527RNA PCR Primer, Index 11 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTT800.045.1549841
TACCCAG206.9178554E-445.1549841
GTCCCCA453.7289283E-1045.154981
ACCCTAT302.2104523E-644.87122
CATTAGT207.13607E-444.87122
CTATTCG253.959526E-544.87122
ACGTAAC600.044.87122
TCCCAAT253.959526E-544.87122
CCATAAA453.965397E-1044.8711972
CTAGATT207.137612E-444.8692363
CAGCACA407.003109E-944.8692364
GCTTCCT253.9605515E-544.8692324
TATTCGG253.9605515E-544.8692323
TTAACGT253.9605515E-544.8692324
CGTAACC600.044.8692323
ATTAAAC650.044.869233
TTAAACC650.044.869234
AGCACAC351.2417513E-744.8672685
CATGACA207.139154E-444.8672687
GCACACT351.2417513E-744.8672686