FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004879305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16201390
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT216960713.391486779838027RNA PCR Primer, Index 14 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC208903712.894183770651777RNA PCR Primer, Index 14 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT11053266.822414619980137RNA PCR Primer, Index 14 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT4205322.595653829702266RNA PCR Primer, Index 8 (97% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4146742.5594964382685683RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC3960572.444586544734742RNA PCR Primer, Index 14 (100% over 42bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTC3381762.0873270750225754RNA PCR Primer, Index 14 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2877331.7759772463967598RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC1844061.1382109806627703RNA PCR Primer, Index 14 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC1404810.867092268009103RNA PCR Primer, Index 14 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT1301870.803554509829095RNA PCR Primer, Index 8 (97% over 36bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCAT734610.4534240580592159RNA PCR Primer, Index 14 (100% over 41bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG708920.4375673939087943RNA PCR Primer, Index 14 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACAGTTC649960.4011754546986401RNA PCR Primer, Index 14 (97% over 38bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC612370.37797374175919474RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACAGTTCC585170.36118505881285495RNA PCR Primer, Index 14 (97% over 39bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACAGTTCCATCTCGT579900.35793225149200164RNA PCR Primer, Index 14 (97% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC518040.31975034240889205RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG482750.29796826074799754RNA PCR Primer, Index 14 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC481950.2974744759554582RNA PCR Primer, Index 14 (100% over 39bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT462560.2855063670462843RNA PCR Primer, Index 14 (100% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT456190.28157460563568926RNA PCR Primer, Index 8 (97% over 36bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT455470.2811301993224038RNA PCR Primer, Index 14 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC432210.26677340647932063No Hit
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACAGTT417830.25789762483342477RNA PCR Primer, Index 14 (97% over 37bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT405150.25007113587167523RNA PCR Primer, Index 14 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT399670.24668871004278028RNA PCR Primer, Index 14 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG365760.22575840714901624RNA PCR Primer, Index 14 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT325260.2007605520267088RNA PCR Primer, Index 14 (100% over 46bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT302560.1867494085384032RNA PCR Primer, Index 14 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC302080.18645313766287955RNA PCR Primer, Index 14 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC300430.18543470652826702RNA PCR Primer, Index 14 (100% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT294460.1817498375139417RNA PCR Primer, Index 14 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT281730.1738924870026584RNA PCR Primer, Index 14 (100% over 46bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC256430.15827654293859972RNA PCR Primer, Index 14 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC245740.1516783436482919RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT239580.14787620074573848RNA PCR Primer, Index 14 (100% over 43bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC235920.1456171353198707RNA PCR Primer, Index 14 (100% over 42bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC227810.14061139198550246RNA PCR Primer, Index 14 (97% over 44bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT216880.13386505725743286RNA PCR Primer, Index 14 (100% over 46bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC215880.1332478262667586RNA PCR Primer, Index 1 (100% over 31bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC205980.12713723945908345RNA PCR Primer, Index 14 (100% over 44bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC193930.11969960602145864RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG193480.11942185207565524RNA PCR Primer, Index 14 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACAGTTCCATCTC187380.11565674303254227RNA PCR Primer, Index 14 (97% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG181390.11195952939840348RNA PCR Primer, Index 14 (100% over 45bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT180980.11170646469222702RNA PCR Primer, Index 14 (100% over 43bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCT177790.10973749783197614RNA PCR Primer, Index 14 (97% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCG176890.10918198994036932RNA PCR Primer, Index 14 (100% over 45bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATC175210.10814504187603656RNA PCR Primer, Index 14 (100% over 42bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGT168170.1037997357016898RNA PCR Primer, Index 14 (100% over 46bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCCG168000.10369480643327518RNA PCR Primer, Index 14 (98% over 51bp)
CCAGTGTGAAAGTAGGTTATCGTCAGGCTTGGAATTCTCGGGTGCCAAGG165950.10242948290239294Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph