FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10585880
Sequences flagged as poor quality0
Sequence length35-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT185198817.494889418735145RNA PCR Primer, Index 16 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC175722916.599744187540384RNA PCR Primer, Index 16 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT9168378.660942689696085RNA PCR Primer, Index 16 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC4427144.182118066707727RNA PCR Primer, Index 5 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT3778493.5693678749428486RNA PCR Primer, Index 16 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC3642983.441357733131303RNA PCR Primer, Index 16 (100% over 38bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC3507773.31363098769304RNA PCR Primer, Index 16 (100% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC2749972.5977717487823404RNA PCR Primer, Index 16 (100% over 51bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2495662.3575366431510654RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC1324571.2512611138611054RNA PCR Primer, Index 16 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG1323621.2503636920123788RNA PCR Primer, Index 7 (97% over 36bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC708300.6690988373191459RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT630290.595406333720012RNA PCR Primer, Index 16 (100% over 41bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACCCGTC599010.5658575385324602RNA PCR Primer, Index 16 (97% over 38bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG583630.5513287511288623RNA PCR Primer, Index 16 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC572240.5405691354899167RNA PCR Primer, Index 16 (100% over 39bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT511900.48356867827710115RNA PCR Primer, Index 16 (100% over 43bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACCCGTCC509870.4816510294845587RNA PCR Primer, Index 16 (97% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC452200.4271727999939542No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC451060.42609589377548207RNA PCR Primer, Index 2 (100% over 34bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACCCGTCCATCTCGT432620.40867646336440616RNA PCR Primer, Index 16 (97% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG401930.3796850143776427RNA PCR Primer, Index 7 (97% over 36bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT377150.35627647394453743RNA PCR Primer, Index 16 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACCCGT352870.33334026080023577RNA PCR Primer, Index 16 (97% over 37bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT317120.29956885965078006RNA PCR Primer, Index 16 (100% over 46bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT273520.25838192006710825RNA PCR Primer, Index 16 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT245010.23144981805952836RNA PCR Primer, Index 16 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC244130.23061852203123406RNA PCR Primer, Index 16 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCCG236130.22306128541037684RNA PCR Primer, Index 16 (98% over 51bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC208740.19718719652971695RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC206270.19485389972302727RNA PCR Primer, Index 16 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC193130.1824411385732693RNA PCR Primer, Index 1 (100% over 31bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC192160.18152482363299036RNA PCR Primer, Index 16 (97% over 44bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC185290.17503504668482925RNA PCR Primer, Index 5 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACCCGTCCATCTCGTATGCC170190.16077076256296122RNA PCR Primer, Index 16 (98% over 51bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT165150.1560097034918212RNA PCR Primer, Index 16 (97% over 43bp)
ACCACGTTCCCGTGGTGGAATTCTCTGGTGCCAAGGAACTCCAGTCACCCG151450.1430679357786032RNA PCR Primer, Index 16 (97% over 36bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACCCGTCCATCTC144350.1363608882775924RNA PCR Primer, Index 16 (97% over 44bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC142470.13458493767169097RNA PCR Primer, Index 16 (100% over 50bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGT136720.12915317385044986RNA PCR Primer, Index 16 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG133350.12596968792391375RNA PCR Primer, Index 16 (100% over 45bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT133150.1257807570083923RNA PCR Primer, Index 16 (100% over 43bp)
CACCACGTTCCCGTGGTGGAATTCTTGGGTGCCAAGGAACTCCAGTCACCC132060.12475108351880052RNA PCR Primer, Index 16 (97% over 35bp)
GAATTCCACCACGTTCCCGTGATGGAATTCTCGGGTGCCAAGGAACTCC128770.12164316995847298RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC123600.116759305792244No Hit
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC120870.11418039879537649RNA PCR Primer, Index 16 (100% over 42bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC118560.11199824672110396RNA PCR Primer, Index 16 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA117460.1109591266857361No Hit
TCCACCACGTTCCCGTGGTGGAATTTTCGGGTGCCAAGGAACTCCAGTCAC116920.11044901321382823RNA PCR Primer, Index 1 (96% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGTAATTCTCGGGTGCCAAGGAACTCC115280.10889977970655251RNA PCR Primer, Index 1 (96% over 27bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCT111150.10499835630103498RNA PCR Primer, Index 16 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)