FastQCFastQC Report
Wed 8 May 2024
EGAF00004879310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538550
Total Bases529.3 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC180818617.157825317524708RNA PCR Primer, Index 17 (100% over 42bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCT174161916.52617295548249RNA PCR Primer, Index 17 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCG9154038.68623292578201RNA PCR Primer, Index 17 (100% over 45bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG4038803.832405786374786RNA PCR Primer, Index 6 (100% over 34bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG3452043.2756308979888127RNA PCR Primer, Index 17 (100% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT3296323.127868634679344RNA PCR Primer, Index 17 (100% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT2972202.820312092270758RNA PCR Primer, Index 17 (100% over 35bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2180482.069051245190278RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC2057261.9521281390703655RNA PCR Primer, Index 17 (100% over 50bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG1298231.2318867396368571RNA PCR Primer, Index 17 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1170501.1106841073961786RNA PCR Primer, Index 17 (100% over 35bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC646880.6138225847009313RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTC609360.5782199638470188RNA PCR Primer, Index 17 (100% over 44bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG600470.5697842682342447RNA PCR Primer, Index 17 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC575340.5459384829981354No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG534280.5069767662534219RNA PCR Primer, Index 17 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGT483790.4590669494380157RNA PCR Primer, Index 17 (97% over 35bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGTAG478850.45437939754520307RNA PCR Primer, Index 17 (97% over 37bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGTAGAGATCTCG444870.42213587258209145RNA PCR Primer, Index 17 (97% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC443720.4210446408661533RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC386270.3665304999264605RNA PCR Primer, Index 17 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGT348700.33088043421533325RNA PCR Primer, Index 17 (97% over 35bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT344360.32676222060909704RNA PCR Primer, Index 17 (100% over 35bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGT313660.29763107827926993RNA PCR Primer, Index 17 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC254290.2412950548225325RNA PCR Primer, Index 17 (100% over 42bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG253460.24050747019276844RNA PCR Primer, Index 17 (100% over 37bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGT252310.23941623847683033RNA PCR Primer, Index 17 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA224390.2129230302081406RNA PCR Primer, Index 1 (100% over 32bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC214820.20384208453724662RNA PCR Primer, Index 17 (98% over 50bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAGCTCG204890.19441953589440672RNA PCR Primer, Index 17 (97% over 45bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACGTAGAGATCT201650.19134510914689404RNA PCR Primer, Index 17 (97% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCT197680.18757798748404667RNA PCR Primer, Index 17 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA190610.18086928467388777No Hit
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC183280.17391386860621244RNA PCR Primer, Index 17 (100% over 42bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT179570.17039346020088153RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAGCT175050.16610444510867245RNA PCR Primer, Index 17 (97% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC156350.1483600685103738No Hit
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG154880.14696518970826156RNA PCR Primer, Index 6 (100% over 34bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAACT150370.14268566358749543RNA PCR Primer, Index 17 (97% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACGTAGAGATCT148920.14130976272826906RNA PCR Primer, Index 17 (97% over 43bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC144500.1371156373504894RNA PCR Primer, Index 17 (100% over 42bp)
ACCACGTTCCCGTGGTGGAATTCTCTGGTGCCAAGGAACTCCAGTCACGT139750.1326083759150927RNA PCR Primer, Index 17 (97% over 35bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC139220.13210546042861682RNA PCR Primer, Index 17 (97% over 42bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCG134530.1276551328218778RNA PCR Primer, Index 17 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTC126900.1204150476109142RNA PCR Primer, Index 17 (100% over 44bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG121330.11512969051719639RNA PCR Primer, Index 17 (100% over 37bp)
TTCCCTTTGTCATCCTATGCCTGTGTAATTCTCGGGTGCCAAGGAACTCC119220.11312751754273596RNA PCR Primer, Index 1 (96% over 27bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATC118510.11245380057028717RNA PCR Primer, Index 17 (100% over 42bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCT115300.1094078407371033RNA PCR Primer, Index 17 (100% over 43bp)
CGTGGTGGAATTCTCAGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCG112280.10654217136133529RNA PCR Primer, Index 17 (97% over 45bp)
TCCCGTGGTGGAATTCTCGGGTGCCTAGGAACTCCAGTCACGTAGAGATC108200.10267067101261558RNA PCR Primer, Index 17 (97% over 42bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAACTCG107320.10183564152563683RNA PCR Primer, Index 17 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph