FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9377025
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT125304513.362926941114054RNA PCR Primer, Index 21 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC123650213.186506381288307RNA PCR Primer, Index 21 (100% over 44bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC6396296.821235946368917RNA PCR Primer, Index 21 (100% over 38bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT6302546.721257541704325RNA PCR Primer, Index 21 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT6058876.461399004481698RNA PCR Primer, Index 21 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT3686593.931513459759359RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC1981662.1133141908014537RNA PCR Primer, Index 21 (100% over 51bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC1879192.0040364614576585RNA PCR Primer, Index 21 (100% over 42bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT1451081.5474844100340992RNA PCR Primer, Index 9 (97% over 36bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1326941.4150970057134327RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGTTTC1099971.1730479549750588RNA PCR Primer, Index 21 (97% over 38bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG783500.8355528539168874RNA PCR Primer, Index 21 (100% over 39bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC756450.8067057515576636RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG712980.7603477648827853RNA PCR Primer, Index 21 (100% over 39bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGTTT703530.7502699416925944RNA PCR Primer, Index 21 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC606320.6466016673731807No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG443800.47328443722822533RNA PCR Primer, Index 21 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG432150.46086045414190535RNA PCR Primer, Index 6 (100% over 34bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGTTTCGATCTCGT307180.32758790767860807RNA PCR Primer, Index 21 (97% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAT304000.32419664019238514RNA PCR Primer, Index 21 (100% over 41bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGTTTCG295660.315302561313423RNA PCR Primer, Index 21 (97% over 39bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG239490.25540083341998127RNA PCR Primer, Index 21 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC212200.22629778634481618RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT209340.22324777847984836RNA PCR Primer, Index 21 (100% over 43bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT205400.21904601939314441RNA PCR Primer, Index 21 (100% over 46bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCCG204730.21833150706114146RNA PCR Primer, Index 21 (98% over 51bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT204440.21802224052937896RNA PCR Primer, Index 21 (100% over 46bp)
ACCACGTTCCCGTGGTGGAATTCTCTGGTGCCAAGGAACTCCAGTCACGTT192710.20551294253774519RNA PCR Primer, Index 21 (97% over 36bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT191700.20443584185815866RNA PCR Primer, Index 21 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT187120.1995515635289444RNA PCR Primer, Index 21 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT186630.19902900973389745RNA PCR Primer, Index 21 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT182340.19445399793644572RNA PCR Primer, Index 21 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG181030.19305696636193248RNA PCR Primer, Index 21 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA171460.1828511708137709No Hit
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT166830.17791357066873556RNA PCR Primer, Index 9 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC159750.17036320154846551No Hit
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT154130.16436982945017212RNA PCR Primer, Index 21 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTGTGTAATTCTCGGGTGCCAAGGAACTCC151950.16204499828037144RNA PCR Primer, Index 1 (96% over 27bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC150130.16010408418448283RNA PCR Primer, Index 21 (97% over 44bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC149700.15964551656842121RNA PCR Primer, Index 21 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCATCACGTTTCGATCTC144060.15363081574379933RNA PCR Primer, Index 1 (96% over 28bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC143930.15349217902266443RNA PCR Primer, Index 21 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCATCACGTTTCGATCTCG133590.14246522751085766RNA PCR Primer, Index 1 (96% over 28bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC131660.14040700542016257RNA PCR Primer, Index 1 (100% over 31bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGC128560.1371010528392534RNA PCR Primer, Index 21 (100% over 50bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC127170.13561870635942636RNA PCR Primer, Index 21 (100% over 42bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC119680.12763109834942318RNA PCR Primer, Index 21 (100% over 42bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT119370.12730050309133226RNA PCR Primer, Index 21 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACGTTTCGATCTC112190.11964349033942002RNA PCR Primer, Index 21 (97% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACGTTTCGATCTCGTATGCC108700.11592162759510613RNA PCR Primer, Index 21 (98% over 51bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT105640.11265833246685382RNA PCR Primer, Index 21 (100% over 46bp)
CACGTTCCCGTGGTGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC105080.11206112812965734RNA PCR Primer, Index 21 (97% over 38bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCT104400.11133595143449015RNA PCR Primer, Index 21 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC103370.11023752202857517RNA PCR Primer, Index 21 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT101240.10796601267459563RNA PCR Primer, Index 21 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTTGGGTGCCAAGGAACTCCAGTCACGT101240.10796601267459563RNA PCR Primer, Index 17 (97% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTTTCGGGTGCCAAGGAACTCCAGTCAC101130.10784870467978917RNA PCR Primer, Index 1 (96% over 33bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGT100750.10744345887954868RNA PCR Primer, Index 21 (100% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG99120.10570516768378031RNA PCR Primer, Index 21 (100% over 45bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATC97130.10358295941409988RNA PCR Primer, Index 21 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT150.00852795449.8582945
AACGTAA253.817177E-545.1500361
AACCCAA253.817177E-545.1500361
CACTAGA351.1818338E-745.1500361
TCCCATA2200.045.1500361
TCCCACA5750.045.1500361
CCCATAA1450.045.1500361
ACTGGAC551.8189894E-1245.1500361
CAAACGA206.921444E-445.1500321
TTAATGC206.921444E-445.1500321
CTATACG206.921444E-445.1500321
CGCGACA206.921444E-445.1500321
TTCCCTA406.6265784E-945.1500321
TGAGGGG2100.045.1500321
CACCAAA1200.045.1500321
CCCATAT2200.044.8697282
ACCCAAT253.960168E-544.8697282
CTCACAC207.1370776E-444.8697242
CACGAAC950.044.8697242
AATTACA1200.044.8697242
Produced by FastQC (version 0.11.5)