FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28898629
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCTTGGACTGGCCTGGACTGGCAACCGGACGCCTTGGAATTCTCGGGT9597843.3212094594522115No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT6393232.21229526148109RNA PCR Primer, Index 9 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC4054641.4030561795855436RNA PCR Primer, Index 9 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC3225311.1160771675362178RNA PCR Primer, Index 9 (100% over 51bp)
ACCTGCTTGGACTGGCCTGGACTGGCAACCGGACGCCTTGGAATTCTCGGG3049831.055354563706119No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC2895521.0019575669143335RNA PCR Primer, Index 9 (100% over 44bp)
GGCTTAGTGAGATTTCCGGATCGGCGTGGAATTCTCGGGTGCCAAGGAACT2673200.9250265817108485RNA PCR Primer, Index 1 (100% over 25bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2520890.8723216592731786RNA PCR Primer, Index 6 (100% over 34bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG2432970.8418980706662589RNA PCR Primer, Index 9 (100% over 45bp)
GTGTGGAGCCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2213900.7660917062882118RNA PCR Primer, Index 6 (100% over 34bp)
CGCGGTAGAGCAGGGTTCGACTCCCTGGTGGAGATGGAATTCTCGGGTGCC2136660.7393637947322691No Hit
CGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAACTCC1883150.6516399099763522RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1873820.648411383114403RNA PCR Primer, Index 1 (100% over 27bp)
GTGAACCGGGATGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1802570.6237562342490366RNA PCR Primer, Index 1 (100% over 33bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1774580.6140706536631894RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGATGGTCTAGTGGCTATGATTTCTGGAATTCTCGGGTGCCAAGGA1697790.5874984588369226RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1544810.534561691490624No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG1382350.4783444917058176RNA PCR Primer, Index 9 (100% over 45bp)
GCCGGACCAGACTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1323470.45796982272065573RNA PCR Primer, Index 6 (100% over 34bp)
ACTATCCGGCTGGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1187020.4107530499111221RNA PCR Primer, Index 6 (100% over 34bp)
GTGAACCGGGATGAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1183090.40939312380528503RNA PCR Primer, Index 1 (100% over 31bp)
CGGTGTGTACTATCCGGCTGGGCCTTGGAATTCTCGGGTGCCAAGGAACTC1064010.368187016761245RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1043640.36113823946457807RNA PCR Primer, Index 9 (100% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC1013030.35054604147483953RNA PCR Primer, Index 9 (100% over 37bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC1010450.34965326555803045RNA PCR Primer, Index 9 (100% over 37bp)
ACACGGGTTAGTCGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG947390.32783216117276703RNA PCR Primer, Index 6 (100% over 34bp)
CGGTTCTATTTTGTTGGTTTCTAGGATCTGGAATTCTCGGGTGCCAAGGA945710.32725081871531RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT942000.32596702078842565RNA PCR Primer, Index 9 (100% over 43bp)
GCTACGATCTGCTGAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC937700.3244790609270772RNA PCR Primer, Index 1 (100% over 31bp)
GTGTTGTGTCCGGTGCGTCCCTGACAGTCCTTGGAATTCTCGGGTGCCA902230.31220512225683783No Hit
TCTTTGGTCGTATAACGGTTAGTACACTTGCTTTGGAATTCTCGGGTGCC791660.2739437915895595No Hit
GGTGTGGAGCCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC783520.2711270489683092RNA PCR Primer, Index 1 (100% over 33bp)
CCGGTGCGTCCCTGACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC758370.2624242139653061RNA PCR Primer, Index 1 (100% over 31bp)
GCCGGACCAGACTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG757490.26211970124949524RNA PCR Primer, Index 6 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT739920.2560398280485901RNA PCR Primer, Index 9 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG698480.24170004743131585RNA PCR Primer, Index 9 (100% over 45bp)
ATGGCTTAGTGAGATTTCCGGATCGGCGTGGAATTCTCGGGTGCCAAGGA695820.2407795885403422RNA PCR Primer, Index 1 (100% over 22bp)
GGCCTGGACTGGCAACCGGACGCCTTGGAATTCTCGGGTGCCAAGGAACTC684370.23681746286303065RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC676700.2341633577149975RNA PCR Primer, Index 9 (100% over 44bp)
GTGCGTTGGGCCTTGAGCAGAAGCCCTGGGATGGAATTCTCGGGTGCCA666080.23048844289464387No Hit
ACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAACTC658150.22774436808057574RNA PCR Primer, Index 1 (100% over 26bp)
CGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAACTC654410.22645018903837966RNA PCR Primer, Index 1 (100% over 26bp)
GCCGGACCAGACTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT628290.21741169797363052RNA PCR Primer, Index 9 (100% over 36bp)
CCTGCCGAATGAACTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT622160.21529049007826634RNA PCR Primer, Index 1 (100% over 30bp)
CTTTGGTCGTATAACGGTTAGTACACTTGCTTTGGAATTCTCGGGTGCCA618870.21415202776574627No Hit
TGCCGGACCAGACTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC616050.21317620292644335RNA PCR Primer, Index 1 (100% over 33bp)
CCCTGTTGAGCTTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC605830.20963970297691284RNA PCR Primer, Index 1 (100% over 31bp)
TGCCGGACCAGACTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG604740.20926252245391988RNA PCR Primer, Index 6 (100% over 34bp)
GGTACGGACAAGGGGAATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC551750.1909260124416283RNA PCR Primer, Index 1 (100% over 31bp)
CATATCTGCGAGTGTTTGGGTGTGGAATTCTCGGGTGCCAAGGAACTCC525570.1818667591462557RNA PCR Primer, Index 1 (100% over 27bp)
CGGTGTGTACTATCCGGCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCC517930.17922303511353427RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGATGGTCTAGTGGCTATGATTTCCGCTTTGGAATTCTCGGGTGCC517860.17919881251114023No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG515420.17835448179911925RNA PCR Primer, Index 6 (100% over 34bp)
CAGCGAGCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT514960.17819530469767267RNA PCR Primer, Index 9 (100% over 36bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT512850.17746516625408076RNA PCR Primer, Index 9 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT508050.17580418780420343RNA PCR Primer, Index 9 (100% over 46bp)
GTGTACTATCCGGCTGGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT499970.17300820741357661RNA PCR Primer, Index 1 (100% over 30bp)
GCCGGACCAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC492620.17046483416220196RNA PCR Primer, Index 9 (100% over 37bp)
CGCGGTAGAGCAGGGTTCGACTCCCTGGTGGAGAATGGAATTCTCGGGTGC487430.1686689012132721No Hit
GAGTTTGGCTGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT483550.16732627696628793RNA PCR Primer, Index 9 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA479590.16595596974513913No Hit
CGCGGTAGAGCAGGGTTCGACTCCCTGGTGGAGTGGAATTCTCGGGTGCC476100.16474829999720747No Hit
ACGGACCAAGGAGTCTAACATCTATGGAATTCTCGGGTGCCAAGGAACTCC470460.16279665031860163RNA PCR Primer, Index 1 (100% over 27bp)
GGTGTGTACTATCCGGCTGGGCCTTGGAATTCTCGGGTGCCAAGGAACTCC467650.16182428585106926RNA PCR Primer, Index 1 (100% over 27bp)
TGGCTTAGTGAGATTTCCGGATCGGCGTGGAATTCTCGGGTGCCAAGGAAC464930.16088306472947211RNA PCR Primer, Index 1 (100% over 24bp)
GTGAACCGGGATGAGCTCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTC453140.15680328641196092RNA PCR Primer, Index 1 (100% over 31bp)
CCTCCATCGTTTTTTTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC451840.1563534380817858RNA PCR Primer, Index 1 (100% over 31bp)
CAACCGACTTAGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG448620.15523919837165978RNA PCR Primer, Index 6 (100% over 34bp)
CCCGTCCGGCGCACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT446400.1544709958385915RNA PCR Primer, Index 9 (100% over 36bp)
TCTTCACTAGTATATCGGCTAGTATAACCGCTTTGGAATTCTCGGGTGCC432920.14980641469185269No Hit
GATAACGAACGAGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG422220.14610381689733445RNA PCR Primer, Index 6 (100% over 34bp)
GCTTGGACTGGCCTGGACTGGCAACCGGACGCCTTGGAATTCTCGGGTGCC404560.13999280035049413No Hit
TGCCGGACCAGACTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC402800.13938377491887244RNA PCR Primer, Index 1 (100% over 31bp)
GTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCTGGAATTCTC401420.13890624361453272No Hit
GGGAATCTGACTGTCTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG392990.13598915021193564RNA PCR Primer, Index 6 (100% over 34bp)
GTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCATGGAATTCT386230.13364993889502508No Hit
GTGAACCGGGATGAGCTCAAATTTGAAATCTGGCGTCTTGGAATTCTCGGG383770.13279868743946296No Hit
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC382170.13224502795617052RNA PCR Primer, Index 9 (100% over 44bp)
CCTGCTTGGACTGGCCTGGACTGGCAACCGTGGAATTCTCGGGTGCCAAGG381420.13198550007337717Illumina Small RNA Adapter 2 (100% over 21bp)
AGGCTGTCTGACGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC377800.13073284549242803RNA PCR Primer, Index 9 (100% over 37bp)
CTATCCGGCTGGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG375800.1300407711383125RNA PCR Primer, Index 6 (100% over 34bp)
CTCAAATTTGAAATCTGGCGTCTTGGAATTCTCGGGTGCCAAGGAACTCC375640.12998540518998322RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTTGAGCTTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC374130.12946288905262598RNA PCR Primer, Index 1 (100% over 31bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG369800.12796454807596583RNA PCR Primer, Index 9 (100% over 45bp)
CCTGCTTGGACTGGCCTGGACTGGCAACCGGACGCCTTTAGCATGGAATTC358810.12416159950010086No Hit
CTTTTAACAACGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG353250.12223763279565962RNA PCR Primer, Index 6 (100% over 34bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG351320.12156978104393812RNA PCR Primer, Index 9 (100% over 45bp)
TGCCGGACCAGACTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG346220.11980499144094345RNA PCR Primer, Index 6 (100% over 34bp)
ACATATCTGCGAGTGTTTGGGTGTGGAATTCTCGGGTGCCAAGGAACTCC344280.11913367931745136RNA PCR Primer, Index 1 (100% over 27bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT343090.1187218950767526RNA PCR Primer, Index 9 (100% over 46bp)
GGTGTGTACTATCCGGCTGGGCTGGAATTCTCGGGTGCCAAGGAACTCC341570.11819591856762478RNA PCR Primer, Index 1 (100% over 27bp)
TTGTGCTGTCCGGTGCGCTCTCGACGACCTGGAATTCTCGGGTGCCAAGG334260.11566638680333242Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGCTGTCCGGTGCGCTCTCGACGACCTGGAATTCTCGGGTGCCAAGGA334050.1155937189961503RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC330330.11430646069749537RNA PCR Primer, Index 9 (100% over 44bp)
ACGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAACT330020.11419918917260746RNA PCR Primer, Index 1 (100% over 25bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT328070.11352441667734481RNA PCR Primer, Index 9 (100% over 43bp)
CGGACAAGGGGAATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG327360.11327873028163377RNA PCR Primer, Index 6 (100% over 34bp)
AGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTGGAATTCTCGG326370.11293615347634657No Hit
TTACGGAAGACTAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG325730.11271468968302961RNA PCR Primer, Index 6 (100% over 34bp)
CCTGCTTGGACTGGCCTGGACTGGCAACCGGACGCCGTGGAATTCTCGGGT320430.11088069264462339No Hit
GTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGTTGGCACTAGCCTTG318800.11031665204601922No Hit
CAGAACTGGCGATGCGGGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT313250.10839614571334855RNA PCR Primer, Index 1 (100% over 30bp)
GGCTTAGTGAGATTTCCGGATCGGCGTTGGAATTCTCGGGTGCCAAGGAAC310060.10729228711853424RNA PCR Primer, Index 1 (100% over 24bp)
CTTCACTAGTATATCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC303400.10498767951932944RNA PCR Primer, Index 1 (100% over 33bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT301520.10433712962646083RNA PCR Primer, Index 9 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG300370.10393918687284437RNA PCR Primer, Index 9 (100% over 45bp)
GCAGGTTGGCACTAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC300350.10393226612930323RNA PCR Primer, Index 1 (100% over 33bp)
GGCCTGATGGTCTAGTGGTAGGATTCTCTGGAATTCTCGGGTGCCAAGGA294530.10191832975882696RNA PCR Primer, Index 1 (100% over 22bp)
GGTTTTGTAGTGTAGCGGTTATCACTTCGGATTTGGAATTCTCGGGTGCC292120.10108438016211772No Hit
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT291350.10081793153578324RNA PCR Primer, Index 9 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC289890.10031271725727889RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC452250.055.98645845
TACGCAG254.1589272E-544.4995042
TTATCTA254.1713538E-544.477028
TCTTCAC108400.044.4152031
TTTGTCA234450.044.297186
TGTCATC236000.044.2131738
GTCATCC236050.044.194399
CCTATGC235750.044.1751614
TCCCTTT235150.044.158872
TATCACG178500.044.10394720
TAGTGTA233650.044.0867848
GTAGTGT227350.044.0861367
CTTTGTC235850.044.0436635
TCATCCT237150.043.9800210
CATCCTA237050.043.97980511
TCCCCGA271950.043.9145241
ACGTTCG182150.043.88151624
TTATCAC223500.043.86018819
CGCGACC212900.043.8206751
TTTTGTA40800.043.732923