FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004879319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4654812
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT4048678.697816367234596RNA PCR Primer, Index 11 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC3168366.806633651369809RNA PCR Primer, Index 11 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT2679435.756258254898372RNA PCR Primer, Index 11 (100% over 37bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC1751183.7620853430815253RNA PCR Primer, Index 11 (100% over 44bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG1645323.534664772712625RNA PCR Primer, Index 2 (97% over 35bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG1322712.841597039794518RNA PCR Primer, Index 11 (100% over 45bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1271132.730786979151897RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1253892.6937500375955032RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG864511.8572393471530106RNA PCR Primer, Index 11 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC777191.669648527158562No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC731241.5709334770126056RNA PCR Primer, Index 6 (97% over 36bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC627351.3477450861603002RNA PCR Primer, Index 11 (100% over 42bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC608751.3077864369173233RNA PCR Primer, Index 11 (100% over 39bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT493041.059204968965449RNA PCR Primer, Index 11 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT443720.9532500990372974RNA PCR Primer, Index 11 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG365970.7862186485727028RNA PCR Primer, Index 11 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT325570.6994267437653766RNA PCR Primer, Index 11 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG324010.6960753731837075RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC320810.6892007668623351RNA PCR Primer, Index 11 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA253240.5440391577576065No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT245890.5282490463632044RNA PCR Primer, Index 11 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC231880.49815116056244596RNA PCR Primer, Index 11 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG210510.45224168022253103RNA PCR Primer, Index 11 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC206350.4433046920047469RNA PCR Primer, Index 11 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG201980.4339165577471228RNA PCR Primer, Index 11 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG199270.4280946255187106RNA PCR Primer, Index 11 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT182820.39275485239790564RNA PCR Primer, Index 11 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT174500.3748808759623375RNA PCR Primer, Index 11 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG171050.3674691910221079RNA PCR Primer, Index 11 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT159460.3425702262518873RNA PCR Primer, Index 11 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC152110.3267801148574851RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC144020.3094002507512656RNA PCR Primer, Index 11 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT138350.29721930767558385RNA PCR Primer, Index 11 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT128900.2769177358827811RNA PCR Primer, Index 11 (100% over 37bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC128730.27655252242195816RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC119870.2575184561696584RNA PCR Primer, Index 1 (100% over 33bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT117310.2520187711125605RNA PCR Primer, Index 11 (100% over 46bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG112690.24209355823607914RNA PCR Primer, Index 11 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT109980.23627162600766688RNA PCR Primer, Index 11 (100% over 41bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC104930.2254226379067511RNA PCR Primer, Index 11 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC103980.2233817391550937RNA PCR Primer, Index 11 (100% over 44bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC99750.2142943689240296RNA PCR Primer, Index 6 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA91590.19676412280453No Hit
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT91060.19562551613255272RNA PCR Primer, Index 11 (100% over 43bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC83510.17940574184306476RNA PCR Primer, Index 1 (100% over 27bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC81870.17588250610336142RNA PCR Primer, Index 11 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC81730.17558174207680138RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC81520.17513059603696132No Hit
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC79080.1698887087169149RNA PCR Primer, Index 11 (100% over 44bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT73840.15863154086566764RNA PCR Primer, Index 11 (100% over 43bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA72500.15575279946859294No Hit
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG70400.1512413390701923RNA PCR Primer, Index 11 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC69940.15025311441149503RNA PCR Primer, Index 11 (100% over 39bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC68470.1470950921326146RNA PCR Primer, Index 11 (100% over 42bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC67980.14604241803965443RNA PCR Primer, Index 11 (100% over 44bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC66850.14361482268241982RNA PCR Primer, Index 11 (100% over 42bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT66460.14277698003700257RNA PCR Primer, Index 11 (100% over 43bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC64380.13830848592811051RNA PCR Primer, Index 11 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC64130.1377714073092533RNA PCR Primer, Index 11 (100% over 44bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG63800.13706246353236176RNA PCR Primer, Index 11 (100% over 45bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC61460.13203540765985822RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC60970.13098273356689807No Hit
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG60520.1300159920529551RNA PCR Primer, Index 11 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG59930.12874848651245205RNA PCR Primer, Index 11 (100% over 45bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT59380.12756691355096617RNA PCR Primer, Index 11 (100% over 46bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG53530.11499927386970729RNA PCR Primer, Index 11 (100% over 51bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC53370.11465554355363869RNA PCR Primer, Index 1 (100% over 27bp)
TCTTCGATGGTCTAGTGGTCATGATTTCCGCTTTGGAATTCTCGGGTGCC51710.11108934152442677No Hit
CCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC51130.10984331912867802RNA PCR Primer, Index 11 (100% over 44bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC48410.1039999037555115RNA PCR Primer, Index 11 (100% over 50bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT47800.1026894319254999RNA PCR Primer, Index 11 (100% over 43bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG47450.10193752185909978RNA PCR Primer, Index 11 (100% over 45bp)
GGAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC46560.10002552197596809RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)