FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004879320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8616677
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT6237897.2393220727665675RNA PCR Primer, Index 12 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG6132057.116490498599402RNA PCR Primer, Index 12 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC4071134.724710001314892RNA PCR Primer, Index 12 (100% over 51bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT3710394.306056731614751RNA PCR Primer, Index 1 (97% over 35bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC2829433.2836672420238107RNA PCR Primer, Index 1 (100% over 27bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC2821743.274742687929465RNA PCR Primer, Index 12 (100% over 44bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2524662.9299694069999376RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT1616331.8758159322903714RNA PCR Primer, Index 12 (100% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1601041.8580712727191702No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1587021.8418004991947592RNA PCR Primer, Index 12 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1358941.5771044916735304RNA PCR Primer, Index 12 (100% over 45bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA1216041.411263297904749RNA PCR Primer, Index 12 (100% over 39bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1063221.2339095454082822RNA PCR Primer, Index 12 (100% over 42bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT996711.156722017083848RNA PCR Primer, Index 12 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT766770.8898674048011779RNA PCR Primer, Index 12 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC709520.8234264786761765RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC647860.7518675702942097RNA PCR Primer, Index 12 (100% over 44bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG617090.7161577485148858RNA PCR Primer, Index 12 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT522980.606939310827132RNA PCR Primer, Index 12 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA443070.5142005438987675No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT442320.5133301387530251RNA PCR Primer, Index 12 (100% over 46bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC402500.4671174282150764RNA PCR Primer, Index 12 (100% over 44bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT390960.45372479437258706RNA PCR Primer, Index 12 (100% over 36bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC385570.44746948272518516RNA PCR Primer, Index 12 (100% over 44bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT356390.413604919854835RNA PCR Primer, Index 12 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG349280.40535347907319724RNA PCR Primer, Index 12 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT338020.39228579648511835RNA PCR Primer, Index 12 (100% over 38bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG332790.38621617126880814RNA PCR Primer, Index 12 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG326310.378695870809594RNA PCR Primer, Index 12 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC307660.35705179618546684RNA PCR Primer, Index 1 (100% over 33bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT303690.35244445161400384RNA PCR Primer, Index 12 (100% over 46bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT303290.3519802355362746RNA PCR Primer, Index 12 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG301230.3495895227359688RNA PCR Primer, Index 12 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT275200.3193806614777367RNA PCR Primer, Index 12 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC267720.3106998208241994RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT232650.2699996762092858RNA PCR Primer, Index 12 (100% over 41bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC227970.26456834809985336RNA PCR Primer, Index 12 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT217910.25289331374496227RNA PCR Primer, Index 12 (100% over 46bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC194490.22571346239391357RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG192170.2230210091430838RNA PCR Primer, Index 12 (100% over 45bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA188870.21919122650181735No Hit
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC185140.21486241157699193RNA PCR Primer, Index 12 (100% over 44bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC167900.19485469862686045RNA PCR Primer, Index 12 (100% over 44bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC161700.18765934942205678RNA PCR Primer, Index 12 (100% over 42bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC161680.18763613861817033RNA PCR Primer, Index 12 (100% over 44bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT159850.18551235006255892RNA PCR Primer, Index 12 (100% over 38bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC154640.1794659356501352RNA PCR Primer, Index 1 (100% over 31bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC153250.177852784780026RNA PCR Primer, Index 12 (100% over 44bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC145500.16885859827402142RNA PCR Primer, Index 1 (100% over 33bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT141350.16404235646758025RNA PCR Primer, Index 12 (100% over 43bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT139040.16136150861869372RNA PCR Primer, Index 12 (100% over 43bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC134800.1564408181947635RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC126280.14655301573913004RNA PCR Primer, Index 12 (100% over 42bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC125030.1451023404962261RNA PCR Primer, Index 12 (100% over 44bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT123690.14354721663583306RNA PCR Primer, Index 12 (100% over 43bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC122960.14270002229397713RNA PCR Primer, Index 12 (100% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG120890.14029770409172818RNA PCR Primer, Index 12 (100% over 45bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG118540.1375704346350687RNA PCR Primer, Index 12 (100% over 45bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG117990.13693213752819097RNA PCR Primer, Index 12 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA115120.13360138717048348No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC114880.13332285752384593No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT112840.13095535552742665RNA PCR Primer, Index 12 (100% over 46bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG111890.12985284234281963RNA PCR Primer, Index 12 (100% over 45bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC110880.12868069674655322RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT107690.12497857352666229RNA PCR Primer, Index 1 (97% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC103740.12039443975908577RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC100370.11648341930421668RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC98090.11383738766115986RNA PCR Primer, Index 12 (100% over 44bp)
CCGAACGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC97780.11347762020091968RNA PCR Primer, Index 12 (100% over 42bp)
TCGGTTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT97390.11302500952513365RNA PCR Primer, Index 12 (100% over 41bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG89930.10436737967548279RNA PCR Primer, Index 12 (100% over 45bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT88130.10227840732570108RNA PCR Primer, Index 12 (100% over 43bp)
CCCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC87150.10114107793526438RNA PCR Primer, Index 12 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)