FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004879322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208768
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT9215029.026574019509503RNA PCR Primer, Index 11 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC7881857.7206671755103065RNA PCR Primer, Index 11 (100% over 44bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT7484957.331883729750739RNA PCR Primer, Index 11 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG6204846.077951815537389RNA PCR Primer, Index 2 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC6039715.916198702918902RNA PCR Primer, Index 11 (100% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC2253112.2070341886503835RNA PCR Primer, Index 6 (97% over 36bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1917761.8785420532624504RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1734431.698961128316365RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1530111.4988194461858668No Hit
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT1438381.4089653129545112RNA PCR Primer, Index 11 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG1215981.1911133645117609RNA PCR Primer, Index 11 (100% over 45bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC1116781.0939419918250666RNA PCR Primer, Index 11 (100% over 42bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1034301.0131486972766939RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC953440.9339422739355032RNA PCR Primer, Index 11 (100% over 39bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC838070.8209315756808265RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT820210.8034368103967099RNA PCR Primer, Index 11 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT701160.6868213676713978RNA PCR Primer, Index 11 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG552870.54156387920658RNA PCR Primer, Index 11 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC513410.5029108311600381RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC482300.4724370266813782RNA PCR Primer, Index 11 (100% over 44bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT445120.43601735292642557RNA PCR Primer, Index 11 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA444000.43492025678318874No Hit
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC402460.3942297444706354RNA PCR Primer, Index 11 (100% over 44bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG386220.37832185039370075RNA PCR Primer, Index 11 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT339760.3328119514519284RNA PCR Primer, Index 11 (100% over 46bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC319110.31258424131099855RNA PCR Primer, Index 11 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG317660.31116389362555796RNA PCR Primer, Index 11 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT314370.3079411737047996RNA PCR Primer, Index 11 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG312110.30572739041576813RNA PCR Primer, Index 11 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT305410.2991644045589047RNA PCR Primer, Index 11 (100% over 43bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG271320.26577154069913234RNA PCR Primer, Index 11 (100% over 45bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT258120.25284147901098347RNA PCR Primer, Index 11 (100% over 43bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG253280.2481004563919956RNA PCR Primer, Index 11 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC246530.24148849302873765RNA PCR Primer, Index 11 (100% over 39bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC242690.2377270205376398RNA PCR Primer, Index 11 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT222790.2182339729926275RNA PCR Primer, Index 11 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT217650.2131990853352726RNA PCR Primer, Index 11 (100% over 46bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC190420.1865259353528261RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC188260.1844101070765836RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC180680.1769851171071769RNA PCR Primer, Index 1 (100% over 27bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC166760.1633497793269472RNA PCR Primer, Index 11 (100% over 44bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC164500.16113599603791565RNA PCR Primer, Index 11 (100% over 44bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT163880.1605286749586238RNA PCR Primer, Index 11 (100% over 41bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT162940.15960789783840715RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC152530.14941078100707159RNA PCR Primer, Index 11 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT150000.14693251918350972RNA PCR Primer, Index 11 (100% over 46bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG148330.1452966704699333RNA PCR Primer, Index 11 (100% over 45bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT144940.14197599553638596RNA PCR Primer, Index 11 (100% over 43bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC144330.14137846995837303RNA PCR Primer, Index 1 (100% over 31bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC143880.1409376724008225RNA PCR Primer, Index 11 (100% over 44bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCT136550.13375756996338833RNA PCR Primer, Index 11 (100% over 43bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA125480.12291395004764533No Hit
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC124200.12166012588394604RNA PCR Primer, Index 11 (100% over 44bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC123330.12080791727268167RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG115290.11293233424444556RNA PCR Primer, Index 11 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA109570.10732930751291439No Hit
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC107140.10494900070214153RNA PCR Primer, Index 11 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC106850.10466493116505342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)