FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15771687
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT12864888.156946051490879RNA PCR Primer, Index 12 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG12222647.749735332688254RNA PCR Primer, Index 12 (100% over 37bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC11583847.3447057375663105RNA PCR Primer, Index 12 (100% over 44bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT10027766.3580769768002625RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC3923252.4875271744867877RNA PCR Primer, Index 12 (100% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT3582772.271646653905825RNA PCR Primer, Index 12 (100% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2645821.6775757723317741RNA PCR Primer, Index 2 (100% over 34bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG2346061.4875136692732998RNA PCR Primer, Index 12 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT1975001.2522439736472073RNA PCR Primer, Index 12 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT1674101.0614590563457162RNA PCR Primer, Index 12 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1504320.953810457942768RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA1444910.9161416911202968RNA PCR Primer, Index 12 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1414390.8967905589300624No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1375910.8723924079903437RNA PCR Primer, Index 12 (100% over 42bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT1309590.8303423723790613RNA PCR Primer, Index 12 (100% over 46bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1266440.8029832192333007RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG1086150.6886707807478046RNA PCR Primer, Index 12 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC967570.6134854185224448RNA PCR Primer, Index 12 (100% over 44bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC960820.6092055973466884RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC743720.47155386738273464RNA PCR Primer, Index 12 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC733930.46534654155893407RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT730520.46318443930570014RNA PCR Primer, Index 12 (100% over 38bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC655240.4154533373633398RNA PCR Primer, Index 12 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT651120.41284106132717446RNA PCR Primer, Index 12 (100% over 46bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT649130.41157930663980336RNA PCR Primer, Index 12 (100% over 43bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG605620.3839918963646692RNA PCR Primer, Index 12 (100% over 45bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG595000.37725831104814594RNA PCR Primer, Index 12 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT592520.37568587304579404RNA PCR Primer, Index 12 (100% over 43bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG571230.36218700003366794RNA PCR Primer, Index 12 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG522230.3311186685355853RNA PCR Primer, Index 12 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG477120.3025167821299015RNA PCR Primer, Index 12 (100% over 45bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC444470.281815128590873RNA PCR Primer, Index 12 (100% over 44bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT423120.26827821272385133RNA PCR Primer, Index 12 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT414550.2628444249495948RNA PCR Primer, Index 12 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT412620.2616207131171193RNA PCR Primer, Index 12 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA383480.24314456658948405No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT380480.24124242384470349RNA PCR Primer, Index 12 (100% over 38bp)
TGTGCTGTCCGGTGCGCTCTCGACGACCCTGGAATTCTCGGGTGCCAAGG377340.23925151443849985Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC315500.20004201199275637RNA PCR Primer, Index 12 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC309550.19626942888227492RNA PCR Primer, Index 12 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC302600.19186279819019994RNA PCR Primer, Index 12 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT286630.1817370583121514RNA PCR Primer, Index 12 (100% over 46bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG284870.18062113456854678RNA PCR Primer, Index 12 (100% over 45bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC279980.17752064189455447RNA PCR Primer, Index 12 (100% over 44bp)
TGTGCTGTCCGGTGCGCTCTCGACGACCCTTGGAATTCTCGGGTGCCAAGG277480.17593552294057066Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCAATGGTCTAGTGGCTATGATTTCCGCTTTGGAATTCTCGGGTGCC263600.16713494250805255No Hit
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT243770.15456177896505302RNA PCR Primer, Index 12 (100% over 43bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT236220.14977471972402193RNA PCR Primer, Index 1 (100% over 30bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT226260.14345960581135042RNA PCR Primer, Index 12 (100% over 41bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC220530.13982651316881953RNA PCR Primer, Index 12 (100% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT212890.13498238964544504RNA PCR Primer, Index 12 (100% over 43bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG211460.13407570160376628RNA PCR Primer, Index 12 (100% over 45bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC205380.13022069230767766RNA PCR Primer, Index 12 (100% over 44bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG202530.12841365670013613RNA PCR Primer, Index 12 (100% over 45bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC200540.12715190201276502RNA PCR Primer, Index 12 (100% over 42bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC196760.12475520215434151RNA PCR Primer, Index 1 (100% over 31bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC184800.11717199307848297RNA PCR Primer, Index 12 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG174590.11069836727041311RNA PCR Primer, Index 12 (100% over 45bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG167540.10622833182017878RNA PCR Primer, Index 12 (100% over 45bp)
CCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC164470.10428180574468668RNA PCR Primer, Index 12 (100% over 44bp)
TCGGTTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT158850.10071845833613106RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)