FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12082113
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC10605418.77777752947684RNA PCR Primer, Index 13 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7787506.445478535087363RNA PCR Primer, Index 1 (100% over 33bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT6923915.730711176099743RNA PCR Primer, Index 13 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC5763904.770605936229863RNA PCR Primer, Index 13 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC4758553.938508106984267RNA PCR Primer, Index 13 (100% over 51bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC3148142.6056203910690123RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT2865452.371646416483607RNA PCR Primer, Index 8 (97% over 36bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA2321361.9213195572661834RNA PCR Primer, Index 13 (100% over 39bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1896651.5697999182758844RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC1527781.2644973606851715RNA PCR Primer, Index 13 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG1481291.2260189918766693RNA PCR Primer, Index 13 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1257631.0409023653395726No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT1090320.9024249317979396RNA PCR Primer, Index 13 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC978010.8094693370273891RNA PCR Primer, Index 1 (100% over 33bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT930050.7697742936190052RNA PCR Primer, Index 13 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT888900.7357156815202771RNA PCR Primer, Index 13 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC767110.6349137770851837RNA PCR Primer, Index 13 (100% over 37bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG654980.542107162877884RNA PCR Primer, Index 13 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC619550.5127828220113485RNA PCR Primer, Index 13 (100% over 44bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA606710.5021555418327903RNA PCR Primer, Index 13 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC556480.4605816879878545RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC527050.43622336589634614RNA PCR Primer, Index 1 (100% over 31bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC467810.3871922071909111RNA PCR Primer, Index 13 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT438660.36306563264223735RNA PCR Primer, Index 13 (100% over 46bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC422110.3493676975211207RNA PCR Primer, Index 13 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA411300.34042058702811334No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG405420.33555388862858676RNA PCR Primer, Index 13 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT399830.33092721446985307RNA PCR Primer, Index 13 (100% over 43bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG391600.3241154920501075RNA PCR Primer, Index 13 (100% over 45bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG382490.3165754202100245RNA PCR Primer, Index 13 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT351820.29119078757167727RNA PCR Primer, Index 13 (100% over 43bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG320730.26545853361907806RNA PCR Primer, Index 13 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG320620.2653674899415359RNA PCR Primer, Index 13 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC316580.2620237039663509RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT298680.24720841462085316RNA PCR Primer, Index 13 (100% over 41bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT292880.24240792980499354RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT288820.23904759043389184RNA PCR Primer, Index 13 (100% over 46bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC287990.23836062450334639RNA PCR Primer, Index 13 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT284210.23523203267507928RNA PCR Primer, Index 13 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT247310.2046910172086621RNA PCR Primer, Index 13 (100% over 43bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC226470.18744237866340102RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC205050.16971369163655398RNA PCR Primer, Index 13 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC197760.16367997882489593RNA PCR Primer, Index 13 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG196380.1625377945066397RNA PCR Primer, Index 13 (100% over 45bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC195980.16220672658830454RNA PCR Primer, Index 13 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT188800.1562640574541887RNA PCR Primer, Index 13 (100% over 46bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC186380.15426109654826106RNA PCR Primer, Index 13 (100% over 44bp)
CCGGTGCGCTCTCGACGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC182180.15078488340574203RNA PCR Primer, Index 1 (100% over 31bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTC169970.1406790351985617RNA PCR Primer, Index 13 (97% over 39bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT169160.14000862266393305RNA PCR Primer, Index 13 (100% over 43bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT168380.1393630402231795RNA PCR Primer, Index 13 (100% over 43bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC157120.13004347832204516RNA PCR Primer, Index 1 (100% over 27bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC151210.1251519498286434RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC149350.12361248400838495RNA PCR Primer, Index 13 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCG140470.11626277622134473RNA PCR Primer, Index 13 (97% over 39bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC140440.11623794612746959RNA PCR Primer, Index 13 (100% over 42bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG140360.11617173254380256RNA PCR Primer, Index 13 (100% over 45bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC137770.1140280677725825RNA PCR Primer, Index 1 (100% over 27bp)
GCATAGCTGTTGGGACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC134910.11166093215648619RNA PCR Primer, Index 1 (100% over 33bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG129750.10739015600996282RNA PCR Primer, Index 13 (100% over 45bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC125510.1038808360756103RNA PCR Primer, Index 13 (100% over 44bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC122630.10149714706359723RNA PCR Primer, Index 13 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCC750.063.68634445
GTATGCC632700.054.3150845
TTCCCTT400450.044.6154561
TAATCTC2500.044.5804445
ACGCTCG254.1169893E-544.57569524
ATCACAC207.3728204E-444.5747721
GTATCTA207.376879E-444.5697946
CATACCA302.3153625E-644.56979410
TTTGTCA401450.044.4643256
TCCCTTT401700.044.4195062
TTGTCAT402900.044.4093937
TGTCATC403300.044.387458
ATCCTAT403200.044.387412
CCTATGC402900.044.36514314
GTCATCC403850.044.3545889
TAGTGTA159550.044.3323488
TATGCCT399300.044.2907416
TTATCAC154450.044.28396619
CTATGCC403450.044.28256615
TCCTATG404200.044.27758813
Produced by FastQC (version 0.11.5)