FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10835684
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC140547512.970800920366448RNA PCR Primer, Index 19 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT108985510.058017564927143RNA PCR Primer, Index 19 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCC7039546.496627254910719RNA PCR Primer, Index 19 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG5225844.822805833023555RNA PCR Primer, Index 15 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT3626203.3465353917666847RNA PCR Primer, Index 1 (97% over 35bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT1841281.6992743605295246RNA PCR Primer, Index 19 (100% over 46bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG1642051.5154096409603677RNA PCR Primer, Index 19 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1635091.5089864193160303RNA PCR Primer, Index 6 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA1479181.3651007172228353RNA PCR Primer, Index 19 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1469861.3564995066301306RNA PCR Primer, Index 15 (97% over 36bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1235401.140121841869881RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT1195811.1035851543843471RNA PCR Primer, Index 19 (100% over 43bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1186131.0946517081893492RNA PCR Primer, Index 19 (100% over 42bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT992440.9158997253888171RNA PCR Primer, Index 19 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG873950.8065480684006658RNA PCR Primer, Index 19 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC846740.7814365941273297RNA PCR Primer, Index 19 (100% over 44bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG696840.6430973808390869RNA PCR Primer, Index 19 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC629060.5805447999406406No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT549070.5067238948644128RNA PCR Primer, Index 19 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT542380.5005498499217954RNA PCR Primer, Index 19 (100% over 43bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC495030.45685163945349455RNA PCR Primer, Index 19 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC472720.4362622608780396RNA PCR Primer, Index 1 (100% over 33bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT452830.4179062438513342RNA PCR Primer, Index 19 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC450720.4159589740712262RNA PCR Primer, Index 19 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG434160.4006761363657338RNA PCR Primer, Index 19 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA417330.3851441219585215RNA PCR Primer, Index 19 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG415910.383833637082809RNA PCR Primer, Index 19 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA406320.3749832497883844RNA PCR Primer, Index 19 (100% over 38bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG399250.368458511710013RNA PCR Primer, Index 19 (100% over 45bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC367440.3391018047407067RNA PCR Primer, Index 1 (100% over 27bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT366640.33836350340227717RNA PCR Primer, Index 19 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT363280.3352626377808729RNA PCR Primer, Index 19 (100% over 46bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT358900.33122043795297096RNA PCR Primer, Index 19 (100% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG357990.3303806201805073RNA PCR Primer, Index 19 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAATCTCG324260.29925198999897007RNA PCR Primer, Index 19 (97% over 39bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC266590.24602969226492763RNA PCR Primer, Index 19 (100% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT265990.24547596626110546RNA PCR Primer, Index 19 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA259910.2398648760890406No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT250240.23094065866077307RNA PCR Primer, Index 19 (100% over 46bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAATCTC240800.222228702867304RNA PCR Primer, Index 19 (97% over 39bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG238330.21994919748490266RNA PCR Primer, Index 19 (100% over 45bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC233630.21561167712162888RNA PCR Primer, Index 19 (100% over 44bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAT223610.2063644528577984RNA PCR Primer, Index 19 (100% over 41bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC221440.20436181047730814RNA PCR Primer, Index 19 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC217560.20078104898592466RNA PCR Primer, Index 1 (100% over 31bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC205770.18990033301081868RNA PCR Primer, Index 19 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC195860.18075462518102225RNA PCR Primer, Index 19 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAATCTCGTATGCCG158250.14604523350810156RNA PCR Primer, Index 19 (97% over 39bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG155120.14315662952149583RNA PCR Primer, Index 19 (100% over 45bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC153020.1412185885081182RNA PCR Primer, Index 19 (100% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG151530.1398435022652931RNA PCR Primer, Index 19 (100% over 45bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT144190.13306958748520167RNA PCR Primer, Index 19 (100% over 43bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG144100.13298652858462834RNA PCR Primer, Index 19 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG143390.1323312861467721RNA PCR Primer, Index 19 (100% over 45bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC137760.12713549047757391RNA PCR Primer, Index 19 (100% over 42bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT137350.12675711104162876RNA PCR Primer, Index 19 (100% over 46bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC133090.12282565641449122RNA PCR Primer, Index 19 (100% over 44bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC128860.11892188808754482RNA PCR Primer, Index 19 (100% over 44bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC126780.11700230460762792RNA PCR Primer, Index 19 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAACTC125630.11594099643363537RNA PCR Primer, Index 19 (97% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAGCTC121230.11188033907227268RNA PCR Primer, Index 19 (97% over 44bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT120720.11140967196902384RNA PCR Primer, Index 1 (100% over 30bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG118490.10935165698815136RNA PCR Primer, Index 19 (100% over 45bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG118050.10894559125201511RNA PCR Primer, Index 19 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC113090.1043681229537517RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG150.006262705753.9076245
ATATGCC1200.049.41531845
GTATGCC889050.047.27414345
CTTATGC453.3287506E-1045.67569444
TACCACA406.537448E-945.219141
TTCACAG7050.045.219141
GTTTCCA600.045.219131
CAAACGT454.0199666E-1044.81462
TGACCTA253.9890583E-544.81462
CCCATAT1950.044.81462
CTTTCGC700.044.80405425
TCCCAAC551.8189894E-1244.8024023
AGTACAT302.2339973E-644.80198721
TTTCGCC650.044.8017826
GAGTTAT1400.044.8001319
ATAACGC207.191908E-444.80012519
ATTGCTT302.2346358E-644.80012519
TGCCCAC253.997873E-544.7978513
CTAACGG207.193706E-444.7978512
CACTACA2600.044.797857
Produced by FastQC (version 0.11.5)