FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10982044
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC184290216.78104731687471RNA PCR Primer, Index 22 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT133484412.154786486012986RNA PCR Primer, Index 22 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT4453614.05535617959644RNA PCR Primer, Index 12 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG3639223.313791130321459RNA PCR Primer, Index 2 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC3286282.9924119772239117RNA PCR Primer, Index 22 (100% over 51bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT3233822.944643091941719RNA PCR Primer, Index 22 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1893411.7240961700754434RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1729741.575061982996972RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG1542571.4046292293128675RNA PCR Primer, Index 22 (100% over 45bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT1308051.1910806403616667RNA PCR Primer, Index 12 (97% over 36bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT1134751.0332775938613976RNA PCR Primer, Index 22 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1074560.9784699460319044No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG1047420.9537568780456535RNA PCR Primer, Index 22 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT948690.8638555809829208RNA PCR Primer, Index 22 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG940440.8563433182383898RNA PCR Primer, Index 22 (100% over 45bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC846730.7710131192335417RNA PCR Primer, Index 22 (100% over 44bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATC819030.7457901279579648RNA PCR Primer, Index 22 (100% over 42bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT691930.6300557528270694RNA PCR Primer, Index 22 (100% over 43bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG637220.5802380686145493RNA PCR Primer, Index 22 (100% over 39bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC612980.5581656748051638RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT535480.487595933871691RNA PCR Primer, Index 22 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC502840.4578746907224192RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC449030.4088765260820299RNA PCR Primer, Index 22 (100% over 44bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC443610.40394119710319865RNA PCR Primer, Index 22 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG439920.4005811668574629RNA PCR Primer, Index 22 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT436650.3976035790787216RNA PCR Primer, Index 22 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA423760.3858662376512059No Hit
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG408040.3715519624579905RNA PCR Primer, Index 22 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT382400.3482047604252906RNA PCR Primer, Index 22 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT367080.33425471615302216RNA PCR Primer, Index 22 (100% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG354740.32301819224180855RNA PCR Primer, Index 22 (100% over 45bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG352160.3206689028017007RNA PCR Primer, Index 22 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT338660.3083761092197409RNA PCR Primer, Index 22 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC335830.3057991754540411RNA PCR Primer, Index 22 (100% over 38bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT269510.24540968876103575RNA PCR Primer, Index 22 (100% over 43bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC261850.2384346666249015RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC252110.22956564369984314RNA PCR Primer, Index 22 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG216570.19720372637370603RNA PCR Primer, Index 22 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT214250.19509118703221368RNA PCR Primer, Index 22 (100% over 46bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC213400.1943171963252014RNA PCR Primer, Index 22 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC206470.18800689561979536RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC203770.1855483369034034RNA PCR Primer, Index 1 (100% over 31bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC201860.1838091342558817RNA PCR Primer, Index 22 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC201190.18319904746329554RNA PCR Primer, Index 22 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC196480.17891022836914514RNA PCR Primer, Index 22 (100% over 44bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG189000.17209911014743703RNA PCR Primer, Index 22 (100% over 39bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAGCTC185420.1688392434049618RNA PCR Primer, Index 22 (97% over 44bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC165170.15040005303202209RNA PCR Primer, Index 22 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG155100.14123053959718246RNA PCR Primer, Index 22 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAACTC149530.13615862402299608RNA PCR Primer, Index 22 (97% over 44bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC148820.13551211413831524RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC147940.13471080611223193RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC143340.13052215052134195RNA PCR Primer, Index 22 (100% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG141300.12866457282451244RNA PCR Primer, Index 22 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT141150.12852798622915734RNA PCR Primer, Index 22 (100% over 41bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC140730.12814554376216303RNA PCR Primer, Index 22 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA140520.1279543225286659No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC136430.12423006136198325RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG135590.12346517642799465RNA PCR Primer, Index 22 (100% over 45bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT132500.12065149256367941RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT128450.11696365448909146RNA PCR Primer, Index 22 (100% over 43bp)
CCCGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT128150.11669048129838125RNA PCR Primer, Index 22 (100% over 43bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG124230.11312101827310107RNA PCR Primer, Index 22 (100% over 45bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA122740.11176425809257366No Hit
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGT122430.11148197912883977RNA PCR Primer, Index 22 (100% over 46bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCT121180.11034375750088052RNA PCR Primer, Index 22 (100% over 43bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCG121100.11027091131669113RNA PCR Primer, Index 22 (100% over 45bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATC117080.10661039056117422RNA PCR Primer, Index 22 (100% over 42bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC114220.10400613947640347RNA PCR Primer, Index 22 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)