FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16653115
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC391468623.507229728492238RNA PCR Primer, Index 23 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT239690314.39312104672309RNA PCR Primer, Index 23 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT4859912.918318885085463RNA PCR Primer, Index 23 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGT4632152.7815516796707405RNA PCR Primer, Index 23 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG4061112.4386488653924507RNA PCR Primer, Index 6 (100% over 34bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG3370602.0240057190501597RNA PCR Primer, Index 23 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC2883061.7312436742315176RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGTATGCC2449761.4710521124726514RNA PCR Primer, Index 23 (100% over 51bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG1964621.1797312394708137RNA PCR Primer, Index 23 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1466000.8803157847645922RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT1436070.8623431712325291RNA PCR Primer, Index 23 (100% over 43bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC1433240.8606437894652142RNA PCR Primer, Index 23 (100% over 42bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG1356250.8144121985586481RNA PCR Primer, Index 23 (100% over 45bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1322690.7942598126536687RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC1141530.6854753600152284RNA PCR Primer, Index 23 (100% over 44bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT1126480.6764380117473517RNA PCR Primer, Index 23 (100% over 46bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC955770.5739286613945799RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC821640.4933851714829328No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG752150.45165724250387995RNA PCR Primer, Index 23 (100% over 39bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT668250.40127627774143154RNA PCR Primer, Index 23 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT653930.39267728590116624RNA PCR Primer, Index 23 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC623030.3741221987598116RNA PCR Primer, Index 23 (100% over 44bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG590090.35434211557417333RNA PCR Primer, Index 23 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT584110.3507511957973028RNA PCR Primer, Index 23 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT561170.33697599518168225RNA PCR Primer, Index 23 (100% over 43bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT558700.33549278918688785RNA PCR Primer, Index 23 (100% over 43bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC539980.32425164901581477RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC536620.32223400847228884RNA PCR Primer, Index 23 (100% over 44bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAGCTC531890.3193936990166704RNA PCR Primer, Index 23 (97% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG529090.3177123318970655RNA PCR Primer, Index 23 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG463750.27847642918457RNA PCR Primer, Index 23 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT438370.2632360372218651RNA PCR Primer, Index 23 (100% over 46bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG398580.23934260947576477RNA PCR Primer, Index 23 (100% over 45bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT397530.23871209680591288RNA PCR Primer, Index 23 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA388730.23342780014429734No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAACTC381450.22905624563332444RNA PCR Primer, Index 23 (97% over 44bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC347790.20884381090264495RNA PCR Primer, Index 23 (100% over 44bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC320510.19246249125163672RNA PCR Primer, Index 23 (100% over 44bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTG305250.18329904044978973RNA PCR Primer, Index 23 (100% over 38bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC296950.17831498791667505RNA PCR Primer, Index 23 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC278220.16706784286303192RNA PCR Primer, Index 23 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC265070.15917142228345868RNA PCR Primer, Index 23 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG264710.15895524651093804RNA PCR Primer, Index 23 (100% over 45bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC240810.1446035771685958RNA PCR Primer, Index 23 (100% over 44bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC239520.1438289473170635RNA PCR Primer, Index 1 (100% over 27bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT228940.1374757815579848RNA PCR Primer, Index 23 (100% over 46bp)
CCCGTGGTGGAATTCTCGGGTGCCAGGAACTCCAGTCACGAGTGGATCTCG226660.13610666833202076RNA PCR Primer, Index 23 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCC226230.13584845838151RNA PCR Primer, Index 1 (100% over 27bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT218370.1311286206814761RNA PCR Primer, Index 23 (100% over 43bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG216690.13011980040971313RNA PCR Primer, Index 23 (100% over 39bp)
CCCGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT208470.1251837869371586RNA PCR Primer, Index 23 (100% over 43bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC206700.12412092272226548RNA PCR Primer, Index 23 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC204890.12303403897709227RNA PCR Primer, Index 1 (100% over 27bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG200040.1201216709306337RNA PCR Primer, Index 23 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC199070.11953919732134198RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC196090.1177497423154767RNA PCR Primer, Index 1 (100% over 31bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG184260.11064596623514579RNA PCR Primer, Index 23 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGACCTC183920.1104418002277652RNA PCR Primer, Index 23 (97% over 44bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG183320.11008150727356412RNA PCR Primer, Index 23 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCGGTGGAATTCTCGGGTGCCAAGGAACTCC181490.1089826137632509RNA PCR Primer, Index 1 (100% over 27bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC179220.10761950541985689RNA PCR Primer, Index 23 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAGCT177490.10658066073524382RNA PCR Primer, Index 23 (97% over 43bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT171440.10294770678038313RNA PCR Primer, Index 23 (100% over 43bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCC166800.10016144126789492RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)