FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004879337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16519671
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT273218016.538949232100325RNA PCR Primer, Index 2 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC258849615.669174041056872RNA PCR Primer, Index 2 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT13941238.439169278855493RNA PCR Primer, Index 2 (100% over 46bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC4657392.8192994884704423RNA PCR Primer, Index 2 (100% over 42bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG4240182.56674603265404RNA PCR Primer, Index 2 (100% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT4070102.4637899871008324RNA PCR Primer, Index 2 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG3626022.194971074181804RNA PCR Primer, Index 2 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3136851.8988574288192543RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC2265541.3714195639852635RNA PCR Primer, Index 2 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT1520330.9203149384754696RNA PCR Primer, Index 2 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1307670.7915835611980409RNA PCR Primer, Index 2 (100% over 35bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG905600.5481949368119983RNA PCR Primer, Index 2 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT794730.4810810094220399RNA PCR Primer, Index 2 (100% over 41bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC735040.4449483285714346RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG720080.4358924581488336RNA PCR Primer, Index 2 (100% over 45bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACCGATGTATCTCGT679280.4111946297235581RNA PCR Primer, Index 2 (97% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT639960.3873927029176307RNA PCR Primer, Index 2 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT626280.37911166632797955RNA PCR Primer, Index 2 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACCGATG599720.36303386429427076RNA PCR Primer, Index 2 (97% over 38bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACCGATGT580010.35110263394470753RNA PCR Primer, Index 2 (97% over 39bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT565030.34203465674346667RNA PCR Primer, Index 2 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG537090.3251214869836088RNA PCR Primer, Index 2 (100% over 45bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG526500.31871094769381303RNA PCR Primer, Index 2 (100% over 35bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT513800.311023143257514RNA PCR Primer, Index 2 (100% over 46bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT513210.3106659932876387RNA PCR Primer, Index 2 (100% over 39bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT496900.30079291530684843RNA PCR Primer, Index 2 (100% over 43bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC485150.2936801828559419RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC478110.2894185967747179RNA PCR Primer, Index 2 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC423320.2562520766908736No Hit
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT396940.24028323566492332RNA PCR Primer, Index 2 (100% over 46bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC372780.22565824706799548RNA PCR Primer, Index 2 (100% over 44bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT364400.22058550681790212RNA PCR Primer, Index 2 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACCGAT353860.2142052344747059RNA PCR Primer, Index 2 (97% over 37bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT350510.21217734905253255RNA PCR Primer, Index 2 (100% over 43bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC344580.20858768918581974RNA PCR Primer, Index 2 (100% over 42bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC306080.1852821403041259RNA PCR Primer, Index 2 (100% over 42bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG295190.1786899993347325RNA PCR Primer, Index 2 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC292370.17698294354651492RNA PCR Primer, Index 2 (100% over 44bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT289890.17548170299517468RNA PCR Primer, Index 2 (100% over 43bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT288910.17488847084182246RNA PCR Primer, Index 2 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGT279810.1693798865606948RNA PCR Primer, Index 2 (100% over 46bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC278030.16830238326174898RNA PCR Primer, Index 2 (97% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG277550.1680118205743928RNA PCR Primer, Index 2 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCG274350.16607473599201825RNA PCR Primer, Index 2 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC239010.1446820581354193RNA PCR Primer, Index 1 (100% over 33bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC238770.14453677679174118RNA PCR Primer, Index 2 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG235980.14284788117148337RNA PCR Primer, Index 2 (98% over 51bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT225990.13680054524088284RNA PCR Primer, Index 2 (100% over 43bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT223820.1354869597584601RNA PCR Primer, Index 2 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAGCTCGT218510.1322726100295823RNA PCR Primer, Index 2 (97% over 46bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC213890.12947594416377906RNA PCR Primer, Index 1 (100% over 31bp)
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACCGATGTATCTC206460.12497827589907813RNA PCR Primer, Index 2 (97% over 44bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT202160.12237531849151233RNA PCR Primer, Index 2 (97% over 43bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG201070.121715499055641RNA PCR Primer, Index 2 (100% over 35bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC200660.12146731009352427RNA PCR Primer, Index 2 (100% over 44bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT193650.11722388418026RNA PCR Primer, Index 2 (100% over 43bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT190990.11561368262116116RNA PCR Primer, Index 2 (100% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT182700.11059542287494709RNA PCR Primer, Index 2 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAACTC176700.1069633892829948RNA PCR Primer, Index 2 (97% over 44bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT171080.10356138448519948RNA PCR Primer, Index 2 (100% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC169430.10256257524741262RNA PCR Primer, Index 2 (100% over 44bp)
CCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC168230.10183616852902216RNA PCR Primer, Index 2 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)