FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11894084
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC171353014.4065738900112RNA PCR Primer, Index 23 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT166754514.01995311282483RNA PCR Primer, Index 23 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT8388497.052657438773763RNA PCR Primer, Index 23 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGT4323753.6352105803187533RNA PCR Primer, Index 23 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTG4101473.4483277568915773RNA PCR Primer, Index 23 (100% over 38bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3505312.94710378705918RNA PCR Primer, Index 6 (100% over 34bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC2422512.03673523745082RNA PCR Primer, Index 23 (100% over 42bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2365221.9885684345259373RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGTATGCC1521731.2794007508270497RNA PCR Primer, Index 23 (100% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1249041.0501355127473455RNA PCR Primer, Index 6 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG861490.7243012576672571RNA PCR Primer, Index 23 (100% over 39bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG668430.5619852693154008RNA PCR Primer, Index 23 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGAGTG644940.5422359552866787RNA PCR Primer, Index 23 (97% over 38bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT593270.4987941904563647RNA PCR Primer, Index 23 (100% over 46bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG591090.4969613464979733RNA PCR Primer, Index 23 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT571950.4808693128449404RNA PCR Primer, Index 23 (100% over 43bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT534630.4494923694838543RNA PCR Primer, Index 23 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG485480.40816930500911214RNA PCR Primer, Index 23 (100% over 45bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC471790.3966593812520578RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC443450.37283240979296933RNA PCR Primer, Index 23 (100% over 44bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGG424920.3572532361466423RNA PCR Primer, Index 23 (100% over 39bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG422340.35508409054450935RNA PCR Primer, Index 6 (100% over 34bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT406670.3419094736509344RNA PCR Primer, Index 23 (100% over 46bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGAGTGGATCTCGT400860.3370246922755884RNA PCR Primer, Index 23 (97% over 46bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAT369790.31090246209796396RNA PCR Primer, Index 23 (100% over 41bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG357410.3004939262241632RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGAGT346680.29147263463079626RNA PCR Primer, Index 23 (97% over 37bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT316170.26582122675441RNA PCR Primer, Index 23 (100% over 46bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGAGTGG313370.2634671152482192RNA PCR Primer, Index 23 (97% over 39bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT299290.25162929738851686RNA PCR Primer, Index 23 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG269280.22639826656680748RNA PCR Primer, Index 23 (100% over 45bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC268170.2254650295054247RNA PCR Primer, Index 23 (100% over 42bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAGCTCGT258080.2169818205420443RNA PCR Primer, Index 23 (97% over 46bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT255580.2148799352686596RNA PCR Primer, Index 23 (100% over 46bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG252990.2127023821254331RNA PCR Primer, Index 23 (100% over 45bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT252510.21229882015294327RNA PCR Primer, Index 23 (100% over 43bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC244600.2056484551479542RNA PCR Primer, Index 23 (100% over 44bp)
CCCGTGGTGGAATTCACGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC241750.20325230593629573RNA PCR Primer, Index 23 (97% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG241030.20264696297756096RNA PCR Primer, Index 23 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC225100.18925375001555395RNA PCR Primer, Index 1 (100% over 33bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT223420.18784128311183948RNA PCR Primer, Index 23 (100% over 46bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC210580.1770460003477359No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAGCTC204170.17165676650677764RNA PCR Primer, Index 23 (97% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT200880.16889068548700345RNA PCR Primer, Index 23 (100% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC198460.16685606054236712RNA PCR Primer, Index 23 (100% over 44bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT184460.15508550301141308RNA PCR Primer, Index 23 (100% over 43bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC172090.14468537467870582RNA PCR Primer, Index 23 (100% over 42bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG171750.1443995182815255RNA PCR Primer, Index 23 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT167600.14091038872770698RNA PCR Primer, Index 23 (100% over 43bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT166820.14025460052241098RNA PCR Primer, Index 23 (100% over 43bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC164930.13866557525573217RNA PCR Primer, Index 23 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG157090.13207406303839792RNA PCR Primer, Index 23 (100% over 45bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT156150.13128375417560528RNA PCR Primer, Index 23 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC152870.12852608069692462RNA PCR Primer, Index 23 (100% over 42bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC150740.1267352744440009RNA PCR Primer, Index 1 (100% over 31bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGAACTC148230.12462498162952272RNA PCR Primer, Index 23 (97% over 44bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT147690.12417097441047163RNA PCR Primer, Index 23 (100% over 43bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGTATGCCG145620.12243061340410913RNA PCR Primer, Index 23 (98% over 51bp)
TCTTTGATGGTCTAGTGGTCATGATTTCCGCTTTGGAATTCTCGGGTGCC143030.12025306026088264No Hit
CCCGTGGTGGAATTCTCGGGTGCCATGGAACTCCAGTCACGAGTGGATCTC141480.11894989139138415RNA PCR Primer, Index 23 (97% over 44bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG141090.11862199728873615RNA PCR Primer, Index 6 (100% over 34bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT140280.11794098646015952RNA PCR Primer, Index 23 (100% over 43bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT139940.11765513006297922RNA PCR Primer, Index 23 (100% over 46bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCG132020.11099635751689663RNA PCR Primer, Index 23 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC130220.1094830001200597RNA PCR Primer, Index 23 (100% over 44bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGT129300.10870950633945413RNA PCR Primer, Index 23 (100% over 46bp)
TCCCGTGGTGGAATTATCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT128410.10796123518212919RNA PCR Primer, Index 23 (97% over 43bp)
CCCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCT127760.1074147450110492RNA PCR Primer, Index 23 (100% over 43bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATC127450.1071541112371495RNA PCR Primer, Index 23 (100% over 42bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTC123510.1038415400462953RNA PCR Primer, Index 23 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)