FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17067566
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT217956212.770198164167052RNA PCR Primer, Index 5 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC211291612.379714834558133RNA PCR Primer, Index 5 (100% over 44bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC10195885.9738336444692814RNA PCR Primer, Index 5 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC7303064.278911240185038RNA PCR Primer, Index 5 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC4088202.395303466235314RNA PCR Primer, Index 5 (100% over 51bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT4069592.3843997439353686RNA PCR Primer, Index 5 (100% over 46bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2825831.6556725194441901RNA PCR Primer, Index 1 (100% over 33bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC2804401.6431165404604264RNA PCR Primer, Index 5 (100% over 35bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC2606451.5271363239491793RNA PCR Primer, Index 1 (100% over 27bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG2511561.4715396442585897RNA PCR Primer, Index 5 (100% over 45bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC2196841.2871431110915288RNA PCR Primer, Index 5 (100% over 42bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG1934041.1331668499187288RNA PCR Primer, Index 5 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1909441.118753546932234No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1876701.0995709640144353RNA PCR Primer, Index 5 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1549770.9080205109504191RNA PCR Primer, Index 5 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT1511810.8857794954476812RNA PCR Primer, Index 5 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC1354470.7935929469966602RNA PCR Primer, Index 5 (100% over 44bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1277460.7484722777694254RNA PCR Primer, Index 1 (100% over 33bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1111790.6514051271282618RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG1042490.6108017979833796RNA PCR Primer, Index 5 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT991420.5808795466207659RNA PCR Primer, Index 5 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT798820.4680339305557688RNA PCR Primer, Index 5 (100% over 43bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG742030.43476029329548216RNA PCR Primer, Index 5 (100% over 45bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC720100.42191136099898485RNA PCR Primer, Index 5 (100% over 44bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC694080.40666607060432636RNA PCR Primer, Index 5 (100% over 44bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT673790.3947780251735954RNA PCR Primer, Index 5 (100% over 43bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG672480.3940104874942332RNA PCR Primer, Index 5 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT658460.385796076605182RNA PCR Primer, Index 5 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT647130.3791577545386378RNA PCR Primer, Index 5 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT643980.37731214866841584RNA PCR Primer, Index 5 (100% over 38bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT626320.3669650376626638RNA PCR Primer, Index 5 (100% over 46bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC619580.36301602700701435RNA PCR Primer, Index 1 (100% over 31bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG589030.3451165795989891RNA PCR Primer, Index 5 (100% over 45bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG588530.34482362628625546RNA PCR Primer, Index 5 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA544800.3192019295545715No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG492520.28857073117514237RNA PCR Primer, Index 5 (100% over 39bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT408980.23962409168360618RNA PCR Primer, Index 5 (100% over 46bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT388690.2277360462528752RNA PCR Primer, Index 5 (100% over 43bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC382310.22399796198239397RNA PCR Primer, Index 5 (100% over 44bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG372500.21825021798656RNA PCR Primer, Index 5 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAT350480.2053485540937706RNA PCR Primer, Index 5 (100% over 41bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC327350.19179653384671255RNA PCR Primer, Index 5 (100% over 44bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC324070.18987476011517987RNA PCR Primer, Index 5 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC318480.1865995420788178RNA PCR Primer, Index 5 (100% over 44bp)
AGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC294040.1722799841523976RNA PCR Primer, Index 5 (100% over 44bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG281760.16508505079165944RNA PCR Primer, Index 5 (100% over 45bp)
CGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG247280.1448829903455478RNA PCR Primer, Index 5 (100% over 45bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG246640.14450801010524875RNA PCR Primer, Index 5 (100% over 45bp)
ACGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC241680.14160191324293106RNA PCR Primer, Index 5 (100% over 44bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT236380.1384966081279545RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGAGCTC223770.13110832558081217RNA PCR Primer, Index 5 (97% over 44bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC222020.13008298898624446RNA PCR Primer, Index 5 (100% over 44bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC214540.1257004074277492RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT213180.12490357441711372RNA PCR Primer, Index 5 (100% over 43bp)
AGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG212140.12429423152662776RNA PCR Primer, Index 5 (100% over 45bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGT205350.12031592553970495RNA PCR Primer, Index 5 (100% over 46bp)
TTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC199960.11715788882843635RNA PCR Primer, Index 1 (100% over 31bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT199450.11685907644944803RNA PCR Primer, Index 5 (100% over 43bp)
CGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC197210.11554664560840133RNA PCR Primer, Index 5 (100% over 44bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC194970.11423421476735462RNA PCR Primer, Index 5 (100% over 42bp)
TCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG191260.11206050118687104RNA PCR Primer, Index 5 (100% over 45bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC190710.11173825254286406RNA PCR Primer, Index 5 (100% over 44bp)
ACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG186240.10911924992702533RNA PCR Primer, Index 5 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC184680.10820523559129638RNA PCR Primer, Index 1 (100% over 27bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG177290.10387538562909321RNA PCR Primer, Index 5 (100% over 45bp)
CCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC171630.10055915412894845RNA PCR Primer, Index 5 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC526700.052.04920245
ATATGCC601.0913936E-1147.83846745
GTCTACG502.1827873E-1145.09961
CTATACG206.9598184E-445.0995981
TTTACAA254.0613122E-544.67874523
CCACTAT207.2890986E-444.67835222
CAATTTG407.2723196E-944.677728
CTACGGC502.3646862E-1144.6775673
CCACATA454.129106E-1044.6775673
TAATTCA1150.044.6732527
TATAGTA407.2795956E-944.6732516
AGCTAGA207.2932016E-444.6732511
TTTGTCA308150.044.572166
TTGTCAT308550.044.550187
GTCATCC308750.044.5430269
TAGTGTA224250.044.523848
CTATGCC308750.044.51408815
TCCCTTT308350.044.509232
ATCCTAT309000.044.5069912
TGTCATC308850.044.5069058