FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004879343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17272005
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT290273316.80599907190856RNA PCR Primer, Index 4 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC265345215.362732931121778RNA PCR Primer, Index 4 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT11488216.651347078697581RNA PCR Primer, Index 4 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG5769263.340237569407836RNA PCR Primer, Index 2 (97% over 35bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC5740313.323476342208099RNA PCR Primer, Index 4 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC4868332.818624705122538RNA PCR Primer, Index 4 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC4838152.8011513428811536RNA PCR Primer, Index 4 (100% over 38bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC3155791.827112717950232RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA2521531.459894204523447RNA PCR Primer, Index 4 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1741701.0083947984035437RNA PCR Primer, Index 2 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC1595360.9236680975949232RNA PCR Primer, Index 4 (100% over 51bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT1123420.6504282508023823RNA PCR Primer, Index 4 (100% over 41bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT954510.5526341614653307RNA PCR Primer, Index 4 (100% over 46bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG940180.5443374987443554RNA PCR Primer, Index 4 (100% over 45bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG929170.537963021664248RNA PCR Primer, Index 4 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG913340.5287979015754106RNA PCR Primer, Index 4 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC790720.4578044066105817RNA PCR Primer, Index 4 (100% over 38bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT769300.4454028353975118RNA PCR Primer, Index 4 (100% over 46bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT723030.4186138204568607RNA PCR Primer, Index 4 (100% over 43bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT715110.4140283655545491RNA PCR Primer, Index 4 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT679000.3931217018522169RNA PCR Primer, Index 4 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC676370.3915990066005655RNA PCR Primer, Index 4 (100% over 44bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT596170.34516548599887503RNA PCR Primer, Index 3 (100% over 34bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT531050.307462856802091RNA PCR Primer, Index 4 (100% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG484140.2803033000511521RNA PCR Primer, Index 2 (97% over 35bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG445430.2578913102445258RNA PCR Primer, Index 4 (100% over 45bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT440550.25506592894108127RNA PCR Primer, Index 4 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC416420.2410953447500739RNA PCR Primer, Index 4 (100% over 42bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT416180.24095639157121598RNA PCR Primer, Index 4 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC396420.22951591317858003RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG385690.22330354814047354RNA PCR Primer, Index 4 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC371200.21491424996692624RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT344880.19967571801884032RNA PCR Primer, Index 4 (100% over 46bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC333950.19334755866501893RNA PCR Primer, Index 4 (100% over 44bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT328120.18997215436192844RNA PCR Primer, Index 4 (100% over 46bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC321240.18598882990133456RNA PCR Primer, Index 4 (100% over 42bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG296420.17161875532111065RNA PCR Primer, Index 4 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC295680.1711903163529654RNA PCR Primer, Index 4 (100% over 44bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT284070.1644684563257132RNA PCR Primer, Index 4 (100% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT282960.16382579787349527RNA PCR Primer, Index 4 (100% over 43bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT279670.16192098137998456RNA PCR Primer, Index 4 (100% over 43bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC269370.1559575741206652No Hit
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG268000.15516438305801789RNA PCR Primer, Index 4 (100% over 45bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC255510.14793302804161995RNA PCR Primer, Index 4 (100% over 44bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC251120.14539134281167704RNA PCR Primer, Index 4 (100% over 42bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC250000.14474289464367338RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACTGACC242110.14017480888871905RNA PCR Primer, Index 4 (97% over 38bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG240450.13921371606828506RNA PCR Primer, Index 4 (100% over 45bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT237130.1372915304274171RNA PCR Primer, Index 4 (100% over 46bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG228770.13245132803053264RNA PCR Primer, Index 4 (100% over 45bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT227740.1318549873046007RNA PCR Primer, Index 4 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACTGACCAATCTCGT226100.1309054739157382RNA PCR Primer, Index 4 (97% over 46bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC225690.13066809556852257RNA PCR Primer, Index 4 (100% over 44bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACTGACCA219000.12679477570785788RNA PCR Primer, Index 4 (97% over 39bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT218010.12622159384506895RNA PCR Primer, Index 4 (100% over 43bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATC213490.12360464230991132RNA PCR Primer, Index 4 (100% over 42bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT196890.11399371410557141RNA PCR Primer, Index 4 (100% over 46bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT188290.10901455852982905RNA PCR Primer, Index 4 (100% over 46bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG183910.1064786630156719RNA PCR Primer, Index 2 (97% over 35bp)
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT182070.10541335531109444RNA PCR Primer, Index 4 (100% over 46bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC177870.10298167468108074RNA PCR Primer, Index 4 (100% over 44bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCCG175480.10159793260828723RNA PCR Primer, Index 4 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)