FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13622892
Sequences flagged as poor quality0
Sequence length42-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT185403413.609694622845133RNA PCR Primer, Index 20 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC180649913.260759903256957RNA PCR Primer, Index 20 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT8232216.042923925404386RNA PCR Primer, Index 20 (100% over 46bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG4895553.5936202092771494RNA PCR Primer, Index 17 (97% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT4752063.4882901516065754RNA PCR Primer, Index 1 (97% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC4609583.3837014930456766RNA PCR Primer, Index 20 (100% over 38bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC3372162.4753627937445297RNA PCR Primer, Index 20 (100% over 42bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2510991.843213614260467RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC1957471.4368975398175365RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC1620071.1892261936745883RNA PCR Primer, Index 20 (100% over 39bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG1602601.1764021912527824RNA PCR Primer, Index 15 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC1471901.0804607421096784RNA PCR Primer, Index 20 (100% over 51bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1254870.9211480205524641No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG712960.5233543655781753RNA PCR Primer, Index 20 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT658970.48372254584415697RNA PCR Primer, Index 20 (100% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG651720.47840062154203383RNA PCR Primer, Index 15 (97% over 36bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG628200.46113556504742165RNA PCR Primer, Index 20 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCAT628090.46105481860973424RNA PCR Primer, Index 20 (100% over 41bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG607510.44594789417694863RNA PCR Primer, Index 20 (100% over 45bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC593820.4358986329774911RNA PCR Primer, Index 20 (100% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT587660.4313768324669975RNA PCR Primer, Index 20 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT538060.3949675296552303RNA PCR Primer, Index 20 (100% over 43bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG508340.3731513103091473RNA PCR Primer, Index 6 (100% over 34bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT472520.3468573339640364RNA PCR Primer, Index 20 (100% over 46bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC456300.334950904697769RNA PCR Primer, Index 20 (100% over 44bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT437040.32081293751723206RNA PCR Primer, Index 20 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT344640.25298592985982715RNA PCR Primer, Index 20 (100% over 46bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA319010.23417200987866602No Hit
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG313240.22993649219270035RNA PCR Primer, Index 20 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC307490.22571565567722332RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG300420.2205258619094976RNA PCR Primer, Index 20 (100% over 45bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT295150.21665737348574737RNA PCR Primer, Index 1 (97% over 35bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT282350.20726142437303327RNA PCR Primer, Index 20 (100% over 46bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGTGGC272420.19997222322543554RNA PCR Primer, Index 20 (97% over 38bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT260520.19123692678470913RNA PCR Primer, Index 20 (100% over 43bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC250960.18421932729115081RNA PCR Primer, Index 20 (100% over 42bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT246730.18111425973280856RNA PCR Primer, Index 20 (100% over 46bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC244940.17980029497407746RNA PCR Primer, Index 20 (100% over 44bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT238780.1752784944635838RNA PCR Primer, Index 20 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC231830.1701767877187898RNA PCR Primer, Index 20 (100% over 42bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG228630.1678278004406113RNA PCR Primer, Index 20 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT224580.16485486341666658RNA PCR Primer, Index 20 (100% over 43bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACGTGG216530.15894569229499875RNA PCR Primer, Index 20 (97% over 37bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC205550.15088572969674868RNA PCR Primer, Index 20 (100% over 44bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG196940.14456548580139958RNA PCR Primer, Index 20 (100% over 45bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATC182870.1342372823626584RNA PCR Primer, Index 20 (100% over 42bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC182310.13382620958897715RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG180360.1323947954663371RNA PCR Primer, Index 20 (100% over 45bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT176720.12972282243740904RNA PCR Primer, Index 20 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCC175520.12884195220809208RNA PCR Primer, Index 1 (100% over 27bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC174680.12822534304757022RNA PCR Primer, Index 20 (100% over 44bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC172460.1265957331233339RNA PCR Primer, Index 20 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGTGGCCATCTCGT170150.12490005793189875RNA PCR Primer, Index 20 (97% over 46bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT164700.12089943897375095RNA PCR Primer, Index 20 (100% over 43bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT163020.11966622065270721RNA PCR Primer, Index 20 (100% over 46bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGTGGCC159890.11736861747123886RNA PCR Primer, Index 20 (97% over 39bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT158550.1163849790485016RNA PCR Primer, Index 20 (100% over 43bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA153100.11238436009035378No Hit
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG141680.10400141174135419RNA PCR Primer, Index 20 (100% over 45bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGT141400.10379587535451357RNA PCR Primer, Index 20 (100% over 46bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT139630.10249659176627107RNA PCR Primer, Index 20 (100% over 43bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC138050.10133677929767043No Hit
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCT136870.1004705902388421RNA PCR Primer, Index 20 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)