FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004879347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534630
Sequences flagged as poor quality0
Sequence length41-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC216564016.000732934701574RNA PCR Primer, Index 7 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT179345513.250860939678441RNA PCR Primer, Index 7 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT10030257.41080472831544RNA PCR Primer, Index 7 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5956974.401280271422269RNA PCR Primer, Index 2 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5013513.7042091287312617RNA PCR Primer, Index 2 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4208753.1096158520772272RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC3024882.234918871073683RNA PCR Primer, Index 7 (100% over 42bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1876581.3865026232708246RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC1779131.3145021326774355RNA PCR Primer, Index 7 (100% over 51bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1720451.2711466807736893RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC1374791.015757357238432RNA PCR Primer, Index 7 (100% over 39bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG859470.635015512060544RNA PCR Primer, Index 7 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG847430.6261198126583438RNA PCR Primer, Index 7 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT737440.5448541999301052RNA PCR Primer, Index 7 (100% over 46bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT725880.5363131463512486RNA PCR Primer, Index 7 (100% over 46bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG715150.5283853345085902RNA PCR Primer, Index 7 (100% over 45bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT692450.5116135424463025RNA PCR Primer, Index 7 (100% over 43bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC645530.47694691321447275RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC618990.45733795456543697RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC582370.43028143362618704RNA PCR Primer, Index 7 (100% over 44bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT580210.42868552742114113RNA PCR Primer, Index 7 (100% over 46bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC565660.41793532590104054RNA PCR Primer, Index 2 (100% over 34bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT517580.3824116359294639RNA PCR Primer, Index 7 (100% over 41bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC451760.33378082740348275No Hit
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT418210.3089925620427008RNA PCR Primer, Index 7 (100% over 43bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT407230.30088003883371767RNA PCR Primer, Index 7 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC377730.2790840976073967RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG353020.2608272261598581RNA PCR Primer, Index 7 (100% over 45bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG337600.24943422908494725RNA PCR Primer, Index 7 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC310870.22968488979750462RNA PCR Primer, Index 7 (100% over 44bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG302830.22374457225650055RNA PCR Primer, Index 7 (100% over 45bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT274000.20244365749192997RNA PCR Primer, Index 7 (100% over 46bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT262910.19424986128176389RNA PCR Primer, Index 7 (100% over 43bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT254970.1883834282872897RNA PCR Primer, Index 7 (100% over 43bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC253050.1869648449939156RNA PCR Primer, Index 7 (100% over 44bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC241920.17874149496513758RNA PCR Primer, Index 7 (100% over 42bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT236560.17478128327113485RNA PCR Primer, Index 7 (100% over 43bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT236490.17472956408856394RNA PCR Primer, Index 7 (100% over 46bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT230310.170163499113016RNA PCR Primer, Index 7 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC225480.16659487551562177RNA PCR Primer, Index 7 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCCG222050.16406063556964617RNA PCR Primer, Index 7 (98% over 51bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC219140.16191059526562604RNA PCR Primer, Index 7 (100% over 44bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG218860.16170371853534232RNA PCR Primer, Index 7 (100% over 45bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT217000.16032946596988615RNA PCR Primer, Index 7 (100% over 43bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC211690.15640619654914836RNA PCR Primer, Index 7 (100% over 44bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC210580.15558607808266647RNA PCR Primer, Index 2 (100% over 34bp)
CGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG200850.14839711170530706RNA PCR Primer, Index 7 (100% over 45bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC197420.14586287175933144RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACC194770.14390493127628903RNA PCR Primer, Index 2 (97% over 34bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC182240.1346471975960924RNA PCR Primer, Index 7 (100% over 42bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACCAGATCATCTCGT179970.13297001838986364RNA PCR Primer, Index 7 (97% over 46bp)
ACGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG178130.13161054273371345RNA PCR Primer, Index 7 (100% over 45bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT169470.12521214100422398RNA PCR Primer, Index 7 (100% over 43bp)
GGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT167410.123690119345708RNA PCR Primer, Index 7 (100% over 46bp)
CCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC166820.12325420052118159RNA PCR Primer, Index 7 (100% over 44bp)
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT165910.12258185114775949RNA PCR Primer, Index 7 (100% over 46bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC157190.11613911869035208RNA PCR Primer, Index 7 (100% over 42bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT156010.11526728104129925RNA PCR Primer, Index 7 (100% over 46bp)
TGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC154860.11441760875620538RNA PCR Primer, Index 7 (100% over 44bp)
GCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG149200.11023574342261297RNA PCR Primer, Index 7 (100% over 45bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACC145250.10731730383468185RNA PCR Primer, Index 2 (97% over 34bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT141490.10453924488515755RNA PCR Primer, Index 7 (100% over 43bp)
GCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG137150.10133265556575982RNA PCR Primer, Index 7 (100% over 45bp)
CGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC136460.10082285219470352RNA PCR Primer, Index 7 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTTC600.053.12024345
TCATCTA150.00931465948.75737443
CCTCATC252.4238265E-548.7573743
TATGCCG45350.047.85453845
GTATGCC253800.047.58331745
ATCTCGT2028250.045.9180645
ATCTCAT2500.045.20049345
TCTCCCA7950.044.9119831
CGCGGCA302.196648E-644.911981
ACGTTAA406.9430826E-944.911981
CTTCAAG253.9383096E-544.911981
TAGCAGC502.1827873E-1144.911981
TCGGATA453.929017E-1044.911981
TGCGCGA253.9383096E-544.911981
ACGGCGC750.044.911981
TCCCATA950.044.911981
TCATCTT1100.044.77904545
TATTGCA7100.044.59571
GCGCGAG254.1593186E-544.498582
AACTTTT502.5465852E-1144.498582
Produced by FastQC (version 0.11.5)