FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11347198
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT187154516.493455036212463RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC171160215.083917633234215RNA PCR Primer, Index 9 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT7619556.714917638698117RNA PCR Primer, Index 9 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG4465703.9355090128858246RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC3913913.449230373877322RNA PCR Primer, Index 9 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC3486463.0725294473578413RNA PCR Primer, Index 9 (100% over 37bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC3428903.0218032680843323RNA PCR Primer, Index 9 (100% over 37bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC2300772.0276106929657876RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC1575271.3882458030608085RNA PCR Primer, Index 9 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1372551.2095937693164427RNA PCR Primer, Index 9 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC1358191.196938662742996RNA PCR Primer, Index 9 (100% over 51bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC634010.5587370556149632RNA PCR Primer, Index 9 (100% over 37bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC632800.5576707130694292RNA PCR Primer, Index 1 (100% over 27bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG579830.510989585270302RNA PCR Primer, Index 9 (100% over 45bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG489540.43141928077751RNA PCR Primer, Index 9 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG484040.4265722692069002RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC482150.4249066597762725No Hit
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT455530.4014471237745213RNA PCR Primer, Index 9 (100% over 36bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC451330.3977457694842374RNA PCR Primer, Index 9 (100% over 37bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT427500.37674499026103186RNA PCR Primer, Index 9 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG413440.36435426613689126RNA PCR Primer, Index 9 (100% over 45bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT403460.35555914332331207RNA PCR Primer, Index 9 (100% over 46bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT396090.349064147818695RNA PCR Primer, Index 9 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT380350.3351928819784408RNA PCR Primer, Index 9 (100% over 43bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT330730.2914640248632306RNA PCR Primer, Index 9 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC294670.25968525445665086RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC288100.25389527881684976RNA PCR Primer, Index 9 (100% over 44bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT257890.22727196617173684RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT249050.21948149666552041RNA PCR Primer, Index 9 (100% over 46bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC237560.20935564885710112RNA PCR Primer, Index 9 (100% over 37bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC222170.19579282920770394RNA PCR Primer, Index 9 (100% over 37bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG218390.19246161034644854RNA PCR Primer, Index 9 (100% over 45bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT214930.18941239943111948RNA PCR Primer, Index 9 (100% over 46bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT213820.18843418436868734RNA PCR Primer, Index 9 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG213470.18812573817783032RNA PCR Primer, Index 9 (100% over 45bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC204150.17991225675272432RNA PCR Primer, Index 9 (100% over 44bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC187000.16479839340073205RNA PCR Primer, Index 9 (100% over 44bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT186910.16471907866594027RNA PCR Primer, Index 9 (100% over 46bp)
TCCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG184810.16286840152079834RNA PCR Primer, Index 6 (100% over 34bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT181330.1598015651088489RNA PCR Primer, Index 9 (100% over 43bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT180660.15921111097206553RNA PCR Primer, Index 9 (100% over 43bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT173110.15255748599786484RNA PCR Primer, Index 9 (100% over 43bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC171870.1514647052074001RNA PCR Primer, Index 9 (100% over 37bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC165290.14566591681928878RNA PCR Primer, Index 9 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC165050.14545441085984398RNA PCR Primer, Index 1 (100% over 31bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG160100.1410921004462952RNA PCR Primer, Index 9 (100% over 45bp)
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACGATC159620.14066908852740562RNA PCR Primer, Index 9 (97% over 37bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC140920.1241892491873324RNA PCR Primer, Index 9 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACGATCAGATCTCGT139320.12277920945770048RNA PCR Primer, Index 9 (97% over 46bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG131710.11607270799363861RNA PCR Primer, Index 9 (98% over 51bp)
TCCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC126980.11190427804291421RNA PCR Primer, Index 9 (100% over 37bp)
CCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT124260.10950721050253993RNA PCR Primer, Index 9 (100% over 46bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTCGT123950.10923401530492374RNA PCR Primer, Index 9 (97% over 46bp)
CGTTCCCGTGGTGGAATTCTCGGGTTCCAAGGAACTCCAGTCACGATC123410.10875812689617297RNA PCR Primer, Index 9 (97% over 37bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAACTC121440.10702201547906365RNA PCR Primer, Index 9 (97% over 44bp)
TCCCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT121250.10685457326116984RNA PCR Primer, Index 9 (100% over 43bp)
CGCGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT120180.10591160919197848RNA PCR Primer, Index 9 (100% over 43bp)
CTGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT119670.10546215902815831RNA PCR Primer, Index 9 (100% over 43bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAGCTC119610.10540928253829711RNA PCR Primer, Index 9 (97% over 44bp)
CGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG119330.10516252558561154RNA PCR Primer, Index 9 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA117910.10391111532556319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)