FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004879350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004879350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10828930
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC153144014.142117457588146RNA PCR Primer, Index 10 (100% over 44bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT151852514.022853596800422RNA PCR Primer, Index 10 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT7485126.912151062016283RNA PCR Primer, Index 10 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT5866365.417303463961813RNA PCR Primer, Index 3 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC5172054.776141317747921RNA PCR Primer, Index 10 (100% over 37bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT4949534.57065471842555RNA PCR Primer, Index 10 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC3618213.3412442411207754RNA PCR Primer, Index 10 (100% over 51bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC2612982.4129623148362764RNA PCR Primer, Index 10 (100% over 42bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1846011.7047021266182345RNA PCR Primer, Index 3 (100% over 34bp)
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC1635771.51055552118261RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT1228141.1341286719925237RNA PCR Primer, Index 10 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC831600.7679429084868035No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC724660.6691889226359391RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT600830.5548378279294446RNA PCR Primer, Index 10 (100% over 39bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT565270.5219998651759684RNA PCR Primer, Index 3 (100% over 34bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG546620.5047774803235408RNA PCR Primer, Index 10 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT448730.41438073752439064RNA PCR Primer, Index 10 (100% over 41bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG370740.3423606949163029RNA PCR Primer, Index 10 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT340670.31459248513010984RNA PCR Primer, Index 10 (100% over 46bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC333340.3078235799843567RNA PCR Primer, Index 1 (100% over 33bp)
CCCGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT296060.2733972793249195RNA PCR Primer, Index 10 (100% over 43bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT290710.2684568096755635RNA PCR Primer, Index 10 (100% over 46bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT280400.25893601676250566RNA PCR Primer, Index 10 (100% over 43bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG259280.23943270480093604RNA PCR Primer, Index 10 (100% over 45bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT252150.23284849010936445RNA PCR Primer, Index 10 (100% over 46bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG250750.23155565693009375RNA PCR Primer, Index 10 (98% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA237520.2193383833859855No Hit
CACGTTCCCGTGGTGGAATTCTCGGTTGCCAAGGAACTCCAGTCACTAGCT237390.21921833459076753RNA PCR Primer, Index 10 (97% over 38bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT220660.20376897809848252RNA PCR Primer, Index 10 (100% over 46bp)
CCCGTGGGAACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT219340.2025500211008844RNA PCR Primer, Index 3 (100% over 34bp)
CCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT201530.1861033361560191RNA PCR Primer, Index 10 (100% over 43bp)
TCCCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC200380.1850413660444753RNA PCR Primer, Index 10 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC196140.18112592841582686RNA PCR Primer, Index 10 (100% over 44bp)
CCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT187880.17349821265812965RNA PCR Primer, Index 10 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG170290.15725468721286406RNA PCR Primer, Index 10 (100% over 45bp)
CCCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC165150.15250814254039874RNA PCR Primer, Index 10 (100% over 44bp)
CCACGTTCCCGTGGTGGAATTCTCGTGTGCCAAGGAACTCCAGTCACTAGC164190.1516216283603274RNA PCR Primer, Index 10 (97% over 37bp)
GCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC163030.1505504237260745RNA PCR Primer, Index 10 (100% over 44bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC162200.14978395834122116RNA PCR Primer, Index 1 (100% over 31bp)
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT155120.143245916263195RNA PCR Primer, Index 10 (100% over 46bp)
CCCGGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC152420.14075259513174432RNA PCR Primer, Index 10 (100% over 42bp)
NCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC150860.13931200958912837RNA PCR Primer, Index 10 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG150740.13920119531661948RNA PCR Primer, Index 10 (100% over 45bp)
NCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT145580.134436181598736RNA PCR Primer, Index 10 (100% over 43bp)
TCCCGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT144130.13309717580591987RNA PCR Primer, Index 10 (100% over 43bp)
CGTGGTGGAATTCTCGGGTGCCAAGTAACTCCAGTCACTAGCTTATCTCGT144040.13301406510153818RNA PCR Primer, Index 10 (97% over 46bp)
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG130680.12067674276221196RNA PCR Primer, Index 10 (100% over 45bp)
TGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT123260.11382472691207719RNA PCR Primer, Index 10 (100% over 43bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC118020.10898583701252108RNA PCR Primer, Index 10 (100% over 50bp)
CCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT112930.10428546495360114RNA PCR Primer, Index 10 (100% over 46bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC110670.10219846282134984RNA PCR Primer, Index 10 (100% over 42bp)
GCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC108520.10021304043889841RNA PCR Primer, Index 10 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)